We narrowed to 4,636 results for: pcr
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Plasmid#61315PurposeProtein expression.DepositorInsertGreen fluorescent protein
TagsGST-tag and His-tagExpressionBacterialAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HTB-kanMX6
Plasmid#26875DepositorInsertHTB tag
UsePcr tagging yeastAvailable SinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-RFP entry
Plasmid#192278PurposeEntry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.DepositorInsertRFP flanked by type V-M CRISPR repeat sequences
UseCRISPRExpressionBacterialPromoterPJ23119Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-BbsI-sgRNAscaffold
Plasmid#159352PurposeExpression vector for a customizable guide RNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC1815_pCR8-gSMN2-DN3
Plasmid#118648PurposeTransient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN3
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-sfGFP-3xP3-RFP
Plasmid#127557PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertsfGFP
UseCRISPRExpressionInsectAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-PuroR [M1G]
Plasmid#171800PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T4 g32
Plasmid#165560PurposeA single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.DepositorInsertT4 gene 32
UseSynthetic BiologyAvailable SinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMaCTag-P19
Plasmid#124789PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreenDepositorInsertmCherry-mNeonGreen
UsePcr templateTagsmCherry-mNeonGreenPromoterNoneAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z25
Plasmid#120068PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LIDDepositorInsertYFP-LID
UsePcr templateTagsYFP-LIDPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template
Plasmid#235608PurposeRepair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPRDepositorInsertRpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
UseCRISPRAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Blunt-U6-HES7-STOP-gRNA
Plasmid#130933PurposegRNA for tag human HES7DepositorAvailable SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR 2.1-TOPO Fzd6 N352A HA
Plasmid#208365PurposeExtracellular HA-tagged Fzd6 N352A (mouse)DepositorAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR 2.1-TOPO Fzd6 S354I HA
Plasmid#208364PurposeExtracellular HA-tagged Fzd6 S354I (mouse)DepositorAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P21
Plasmid#120032PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTagDepositorInsert24xSunTag
UsePcr templateTags24xSunTagPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z20
Plasmid#120063PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntagDepositorInsert10xSuntag
UsePcr templateTags10xSuntagPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z14
Plasmid#120057PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5DepositorInsertmNeptune2.5
UsePcr templateTagsmNeptune2.5PromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z33
Plasmid#120050PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAGDepositorInsertFLAG
UsePcr templateTagsFLAGPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only