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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,331 results for: Set;

Showing: 5401 - 5420 of 8331 results

  1. pST50Trc4-FLAGDHFR

    Plasmid
    #63992
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63992-plasmid-map-sequence-id-177398

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  2. pST50Trc3-HISDHFR

    Plasmid
    #63971
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63971-plasmid-map-sequence-id-177508

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  3. pST50Trc4-DHFRSTR

    Plasmid
    #64004
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64004-plasmid-map-sequence-id-177410

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  4. pST50Trc4-FLAGNDHFR

    Plasmid
    #63997
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63997-plasmid-map-sequence-id-177403

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  5. pTH09

    Plasmid
    #70064
    Purpose
    artificial transcription template "terminator insertion"
    Depositor
    Jeff Gelles
    Article
    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi
    Insert
    lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pST50Trc2-FLAGDHFR

    Plasmid
    #63956
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63956-plasmid-map-sequence-id-177362

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  7. pST50Trc2-FLAGHISNDHFR

    Plasmid
    #63965
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63965-plasmid-map-sequence-id-177498

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  13. pSF2

    Plasmid
    #222590
    Purpose
    gsdA disruption cassette via integration of pyrG from A. nidulans
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    pyrG
    Use
    A. niger
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222590-plasmid-map-sequence-id-443810

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  14. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

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  15. psLIB CMV

    Plasmid
    #229953
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229953-plasmid-map-sequence-id-461575

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  16. psLIB EF1alpha

    Plasmid
    #229954
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229954-plasmid-map-sequence-id-461280

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  17. psLIB TRE

    Plasmid
    #229955
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229955-plasmid-map-sequence-id-461253

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  19. pBH504

    Plasmid
    #215782
    Purpose
    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene
    Depositor
    Corey Wilson
    Article
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bt1311, P(O1), and P1
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215782-plasmid-map-sequence-id-430549

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Showing: 5401 - 5420 of 8331 results