Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,326 results for: puro

Showing: 5401 - 5420 of 18326 results

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pGL3-MTS-CCDB-N136-RsDddA-D-UGI-PURO

Plasmid

#205466

Purpose

A backbone containing a RsDddA fragment D for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-D; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

Plasmid

#204729

Purpose

Cre-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

Plasmid

#204727

Purpose

Frt-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

Plasmid

#198845

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-R18 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro

Plasmid

#198841

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-R15 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

Plasmid

#198840

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-L17 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

Plasmid

#198844

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-L18 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro

Plasmid

#173232

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro

Plasmid

#173233

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro

Plasmid

#173234

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro

Plasmid

#173235

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV puro PRDM14 ΔSET+ΔZinc finger domains

Plasmid

#193678

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Mutation

Deletion of SET and Zinc finger domains

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS

Plasmid

#191365

Purpose

Overexpression of NLS-dPbaCas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPbaCas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS

Plasmid

#191364

Purpose

Overexpression of NLS-dPba5Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba5Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS

Plasmid

#191363

Purpose

Overexpression of NLS-dPba4Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba4Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS

Plasmid

#191366

Purpose

Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm6Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

Plasmid

#191362

Purpose

Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dBba2Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

Plasmid

#191361

Purpose

Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba3Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro

Plasmid

#185450

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal YBBR and Strep tag in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

Strep-Tag II and YBBR

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166134

Purpose

This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting firefly luciferase gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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