We narrowed to 11,691 results for: NSI

1. pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

   Plasmid

   #191369

   Purpose

   Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dPba3Cas13b-3xGFP-NLS
   

   Tags

   3xGFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB078d8

   Plasmid

   #79206

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1+2); Ppro1

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYFP-NGAT

   Plasmid

   #11383

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMVie-IRES-BlastR alt MCS

   Plasmid

   #120862

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAB092a

   Plasmid

   #79217

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1+2); Ppro1

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lyn-RAB-EKARev

   Plasmid

   #118477

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-RAB-EKARev
   

   Tags

   N-terminal targeting sequence from Lyn-kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

   Plasmid

   #200401

   Purpose

   Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Open-loop DART VADAR sensor entry cassette
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-UBC-TagRFP-T-ps

   Plasmid

   #178656

   Purpose

   Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

   Insert

   TagRFP-T with palmitoylation signal at N-terminus
   

   Use

   Lentiviral

   Tags

   Palmitoylation signal

   Promoter

   Ubc

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMALB

   Plasmid

   #161784

   Purpose

   Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

   Depositor

   John Dick, Peter van Galen

   Article

   van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Bidirectional minhCMV and hPGK

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL

    Plasmid

    #161783

    Purpose

    Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Bidirectional minhCMV and hPGK

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pRS1422

    Plasmid

    #12548

    Depositor

    Robert Sobol

    Article

    Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

    Insert

    human Methylpurine DNA glycosylase (MPG Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

    Plasmid

    #191145

    Purpose

    Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3UTR(Bdnf)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-HisD-KRaION2

    Plasmid

    #177800

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Expression

    Bacterial

    Promoter

    araB

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fyn-Fyn Biosensor

    Plasmid

    #124655

    Purpose

    FRET biosensor. Fyn-tagged biosensor targeting to membrane

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_nLightR

    Plasmid

    #217657

    Purpose

    Expresses norepinephrine indicator nLightR under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightR
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-INU-TEX264 LIR4A-FLAG

    Plasmid

    #128259

    Purpose

    Expresses TEX264 LIR4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 LIR4A (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed FEEL (aa 273-276) to alanines

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_human-pro-IL-18_DeltaN24

    Plasmid

    #214327

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Deletion of first 24 amino acids

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac_human-pro-IL-18

    Plasmid

    #214337

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB078d3

    Plasmid

    #79203

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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