We narrowed to 6,018 results for: cki

1. pAAV-CaMKIIa-eArch 3.0-EYFP

   Plasmid

   #35516

   Purpose

   AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   Arch3.0-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Enhanced trafficking signal and ER export signal …

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGV15 (CK2alpha K68M)

   Plasmid

   #27089

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha K68M (kinase dead) (CSNK2A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K68M (kinase dead)

   Promoter

   CMV

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

3. MYH11-gRNA1

   Plasmid

   #132587

   Purpose

   gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

   Depositor

   James Thomson

   Article

   Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

   Insert

   MYH11-gRNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNKX2-5-T2A-GFP-PGK-Puro

   Plasmid

   #203607

   Purpose

   Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

   Depositor

   Matthew Birket

   Article

   Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

   Insert

   NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mini-Ii-Str_SBP-EGFP-LIMP2

   Plasmid

   #222324

   Purpose

   Synchronize the trafficking of LIMP2 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CaMKIIa-eArchT 3.0-EYFP

   Plasmid

   #35513

   Purpose

   Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   eArchT 3.0-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Enhanced trafficking signal and ER export signal …

   Promoter

   CaMKIIa

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. xlox dNGFR-TERT

   Plasmid

   #69805

   Purpose

   MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

   Depositor

   David Ott

   Article

   Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

   Insert

   hTERT reverse transcriptase (TERT Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mutation

   Promoter

   MLV promoter

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBMN-AS-YY1

   Plasmid

   #154943

   Purpose

   Knocking down of human YY1 through shRNA and amiRNA targeting YY1

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   shRNA and amiRNA against YY1 (YY1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

   Plasmid

   #67275

   Purpose

   For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

   Insert

   puromycin resistant gene
   

   Use

   Tags

   linked to red flouresent protein gene (katushka)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2 Δ18 P.1

    Plasmid

    #173476

    Purpose

    Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 Spike Truncated to remove 18 amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-730pUC19-tNGFR-P2A-Coronin1A

    Plasmid

    #186110

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CORONIN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Bmpr2Δ751-813aa

    Plasmid

    #163603

    Purpose

    Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The LIMK-binding region (751-813 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-PS-Grb2 1-154 (XSB382)

    Plasmid

    #78510

    Purpose

    Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Grb2 1-154 (GRB2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-4mtD3cpv

    Plasmid

    #181874

    Purpose

    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    4mtD3cpv
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    the 4mtD3cpv CDS differs from the recorded GeneBa…

    Promoter

    CaMKIIa

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShuttle-EGFP-CLASP2 340-1362

    Plasmid

    #24372

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1362) (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CLASP2 deletion mutant that retains MT lattice bi…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-FHC-POLQ-S1977P

    Plasmid

    #64876

    Purpose

    lentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    S1977P, mimicking the chaos1 mutation

    Promoter

    EF1alpha

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

    Plasmid

    #184334

    Purpose

    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223 LC3B no-stop

    Plasmid

    #123205

    Purpose

    Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223 LC3A no-stop

    Plasmid

    #123204

    Purpose

    Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge