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pAc5.1B-lambdaN-HA-DmCup-L379AL383A_M

Plasmid

#146912

Purpose

Insect Expression of DmCup-L379AL383A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-L379AL383A (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-CS-DmCup_311-440-Y342AL347A_M

Plasmid

#146916

Purpose

Bacterial Expression of DmCup_311-440-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_311-440-Y342AL347A (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat-del57-499_H

Plasmid

#146472

Purpose

Insect Expression of DmHPat-del57-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499 (Patr-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPrdm9#1/Cre

Plasmid

#193231

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgPrdm9#1 (Prdm9 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPrdm9#2/Cre

Plasmid

#193232

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgPrdm9#2 (Prdm9 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #2 GFP

Plasmid

#106951

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #3 GFP

Plasmid

#106952

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgErich3#1/Cre

Plasmid

#193211

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgErich3#1 (4922501L14Rik Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgErich3#2/Cre

Plasmid

#193212

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgErich3#2 (4922501L14Rik Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

Plasmid

#188975

Purpose

A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g1 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-SBP-DmDCP1-dsRNAres_J

Plasmid

#146711

Purpose

Insect Expression of DmDCP1-dsRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmDCP1-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-dsRNAres_H

Plasmid

#146502

Purpose

Insect Expression of DmDCP1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBS10-riboE-dddAI

Plasmid

#186561

Purpose

Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Expression

Bacterial

Promoter

pSpac(hy)

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_771-1132_C

Plasmid

#146030

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_771-1132_C

Plasmid

#146034

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup-Y342AL347A_C

Plasmid

#146035

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-Y342AL347A (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

Plasmid

#176573

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Avitag, Hexahistidine tag, and mEGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q W330Y)-6xHis

Plasmid

#176577

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc180

Plasmid

#161942

Purpose

Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI

Expression

Bacterial

Promoter

PBAD

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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