We narrowed to 11,787 results for: nsf

1. AAV-CAG-K-red-SPOTIT(R271F)

   Plasmid

   #191494

   Purpose

   Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   K-red-SPOTIT(R271F)
   

   Use

   AAV

   Mutation

   R271F in OR

   Promoter

   CAG

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaMKII-Aion-ts-citrine-ER

   Plasmid

   #192576

   Purpose

   AAV-vector for optogenetic long-term silencing of excitatory neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   ts-Citrine-ER

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mDlx-Aion-citrine

   Plasmid

   #192572

   Purpose

   AAV-vector for optogenetic long-term silencing of GABAergic neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   minimal Dlx

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CaMKII-somBiPOLES-ChroME2s-mCerulean

   Plasmid

   #192582

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKII-somBiPOLES-ChroME2f-mCerulean

   Plasmid

   #192581

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. hSyn-Aion-mKate2

   Plasmid

   #192574

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2iC6FAST1nls2ER4(#638)

   Plasmid

   #184068

   Purpose

   cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   YFAST-2nls-ERT2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSmp38ERT(#143)

   Plasmid

   #184077

   Purpose

   cyclofen-inducible P38 activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   5myc-ZfP38a-ERT2
   

   Use

   Synthetic Biology

   Tags

   5xMyc

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSTG4FER3(#190)

   Plasmid

   #184069

   Purpose

   cyclofen-inducible GAL4 activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   Gal4bdFF-ERT2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Clim Act MCH (CAM)

    Plasmid

    #104443

    Purpose

    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Promoter

    actin

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Clim EF1 MCH (CEM)

    Plasmid

    #104444

    Purpose

    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Promoter

    EF1

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Clim Tub MCH (CTM)

    Plasmid

    #104445

    Purpose

    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Promoter

    tubulin promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL2209

    Plasmid

    #137050

    Purpose

    Drug Resistance pDONR221 PUR

    Depositor

    Jeremy Mottram

    Article

    Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

    Insert

    puromycin N-acetyl-transferase
    

    Use

    Leishmania expression gateway vector

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW8_GST

    Plasmid

    #110237

    Purpose

    Cre/Lox C-terminal tagging construct encoding glutathione S-transferase

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid

    #119229

    Purpose

    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.

    Depositor

    Kari Alitalo

    Insert

    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIAGO-pegA1

    Plasmid

    #114455

    Purpose

    Expression of PegA glycosyltansferase in streptomycetes

    Depositor

    Patrick Caffrey

    Article

    Stephens et al Biosci Biotechnol Biochem. 2013;77(4):88

    Insert

    pegA
    

    Expression

    Bacterial

    Promoter

    ermE

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBTet(-ΔrtTA)-GB dTomato-P2A-CaMXMT

    Plasmid

    #139176

    Purpose

    SB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ΔrtTA)-GB backbone.

    Depositor

    Neal Devaraj

    Insert

    CaMXMT
    

    Use

    Transposon

    Tags

    dTomato-P2A

    Expression

    Mammalian

    Mutation

    P131T on dTomato (Please see depositor comments)

    Promoter

    tight TRE-controlled CMV

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-MCS2

    Plasmid

    #46954

    Purpose

    Cloning plasmid for the generation of Knock-In in human cells using rAAV.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

    Plasmid

    #159914

    Purpose

    Mutagenesis of Rosa26 with SauCas9

    Depositor

    Larry Zweifel

    Insert

    Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-minCMV-mCherry

    Plasmid

    #27970

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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