Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,476 results for: pcdna

Showing: 5441 - 5460 of 12476 results

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pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP

Plasmid

#233360

Purpose

CMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_JNK_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP

Plasmid

#233358

Purpose

CMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKC_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

Plasmid

#233355

Purpose

CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP

Plasmid

#233362

Purpose

CMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_AMPK_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-K627M-V5/HIS

Plasmid

#234755

Purpose

Expression of the K627M kinase defective mutant variant of human PDGFRA

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA-K627M receptor tyrosine kinase, full length (PDGFRA Human)

Tags

V5/His

Expression

Mammalian

Mutation

K627M substitution

Promoter

CMV

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3

Plasmid

#233062

Purpose

Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Alfa and HiBiT

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

Plasmid

#233240

Purpose

Parallel coiled-coil with (GGS)3 linker to prone condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)3-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

Plasmid

#233241

Purpose

Parallel coiled-coil with (GGS)12 linker to prevent condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)12-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1 C122E

Plasmid

#232274

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

changed cysteine 122 to glutamic acid

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N2 (1-399)

Plasmid

#225452

Purpose

Express mEGFP-fusion protein of FXR1 fragment (1-399)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N2 fragment (1-399)

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1 (1-379)

Plasmid

#225450

Purpose

Express mEGFP-fusion protein of FXR1 fragment (1-379)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N1 fragment (1-379)

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-N1(1-445)

Plasmid

#225464

Purpose

Express mEGFP-fusion protein of FMR1 fragment (1-445)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N1 fragment (1-445)

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-N2(1-491)

Plasmid

#225465

Purpose

Express mEGFP-fusion protein of FMR1 fragment (1-491)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N2 fragment (1-491)

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-AP4-cyto

Plasmid

#229694

Purpose

Transient expression of EGFP AP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Control construct with tandem AP4 motifs (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-cyto

Plasmid

#229695

Purpose

Transient expression of FP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

Plasmid

#228533

Purpose

A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:FRB:gs10:Cul1(dC501) (CUL1 Synthetic, Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:Rbx1:gs10:FRB:HA

Plasmid

#228534

Purpose

Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Rbx1:gs10:FRB:HA (RBX1 Synthetic, Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaE

Plasmid

#228826

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaE

Tags

eGFP

Expression

Mammalian

Mutation

aa 49-494

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaD

Plasmid

#228825

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaD

Tags

eGFP

Expression

Mammalian

Mutation

aa 30-700

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaC

Plasmid

#228824

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 5441 - 5460 of 12476 results

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