We narrowed to 15,323 results for: pET
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Plasmid#133973PurposeHeterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.DepositorInsertMrBBS:StrepII
UseSynthetic BiologyTagsStrepIIExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-B-eSpCas9
Plasmid#126772PurposeExpression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a SznF E281A
Plasmid#139591Purposeexpresses SznF E281ADepositorInsertSznF E281A
TagshexahistidineExpressionBacterialMutationchanged glutamate 281 to alaninePromoterT7Available SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a SznF H225A
Plasmid#139590Purposeexpresses SznF H225ADepositorInsertSznF H225A
TagshexahistidineExpressionBacterialMutationchanged histidine 225 to alaninePromoterT7Available SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SznF E215A
Plasmid#139565Purposeexpresses SznF E215A in E. coliDepositorInsertSznF E215A
TagshexahistidineExpressionBacterialMutationchanged glutamate 215 to alaninePromoterT7Available SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-NT-G46D
Plasmid#134588PurposeExpress human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coliDepositorInsertDDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (G154D)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-FL-G84D
Plasmid#134577PurposeExpress human DDX43 mutant (full-length with G84D mutation) in E. coliDepositorInsertDDX43-G84D (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (G84D)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 D353A
Plasmid#133016PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationD353APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 C351A
Plasmid#133014PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationC351APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-GreBdm-D41N
Plasmid#129691Purposeexpress E. coli GreB E82C/C68S/D41NDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only