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pWJPrP38

Plasmid

#22110

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP genomic vector with cloning sites

Use

Construction of targeting vector

Mutation

2.2 Kb EcoR1 fragement isolated from pWJPrP34 and…

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
SMAD4 KO Hyg

Plasmid

#16645

Depositor

Bert Vogelstein

Article

Zawel et al Proc Natl Acad Sci U S A. 2002 Mar 5. 99

Insert

Smad4 knock-out construct (SMAD4 Human)

Expression

Bacterial

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
CaMCLC-His

Plasmid

#111292

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

(S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase

Tags

His tag, GGGSGGGSLEHHHHHH

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA1

Plasmid

#111296

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA3

Plasmid

#111297

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA3 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pSL3 - Brr2 del113 N1104L

Plasmid

#90253

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 113 amino acids and amino acid N110…

Availability

Academic Institutions and Nonprofits only

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pSL7 - Brr2 del247 N1104L

Plasmid

#90257

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids and N1104L

Availability

Academic Institutions and Nonprofits only
pSL11 - Brr2 del422 N1104L

Plasmid

#90261

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid N110…

Availability

Academic Institutions and Nonprofits only

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pSL12 - Brr2 del422 Q904E

Plasmid

#90262

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid Q904E

Availability

Academic Institutions and Nonprofits only

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pSL13 - Brr2 del422 R1107L

Plasmid

#90263

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid R110…

Availability

Academic Institutions and Nonprofits only

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NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE57

Plasmid

#90402

Purpose

Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ISRE

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgTP53

Plasmid

#187819

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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mCardinal-C1

Plasmid

#54799

Purpose

Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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hSyn-PreSynBar-hSyn-PreRNA short

Plasmid

#247715

Purpose

SynBar protein and RNA construct used in HEK and neuron culture experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

PreSynBar and PreRNA short

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-PostSynBar-hSyn-PostRNA short

Plasmid

#247716

Purpose

SynBar protein and RNA construct used in HEK and neuron culture experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

PostSynBar and PostRNA short

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/intron/GFP

Plasmid

#113547

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

GFP

Tags

GFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn-PreSynBar-hSyn-PreRNA barcode landing pad

Plasmid

#247717

Purpose

The set of SynBar constructs used in the optimized connectome-seq experiments

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

PreSynBar and PreRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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