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Fibronectin-human-plasma in pMAX

Plasmid

#120401

Purpose

enables eukaryotic expression of human plasma fibronectin

Depositor

Inaam Nakchbandi

Article

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

Fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-GATA1-IRES-Puro

Plasmid

#61062

Purpose

lentiviral vector for GATA1

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-kanMX6

Plasmid

#39292

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCalpha EV (PKA catalytic subunit Calpha)

Plasmid

#15310

Depositor

Stanley McKnight

Article

Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

Insert

PRKACA (Prkaca Mouse)

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
MXS_BidirectionalCAG

Plasmid

#78175

Purpose

Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

Bidirectional CAG Promoter

Use

Synthetic Biology

Expression

Mammalian

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-2XboxB

Plasmid

#75391

Purpose

sgRNA1-2XboxB

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XboxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-Rhotekin(8-89)-mCherry-C1

Plasmid

#29675

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

Insert

Rhotekin (Rtkn Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

aa 8-89

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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MD-2

Plasmid

#20864

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Myeloid differentiation factor 2 (LY96 Human)

Tags

protein A tag

Expression

Insect

Mutation

contains amino acids 19–160

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pBT224_(pCA-tTA2)

Plasmid

#36430

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tetracycline transactivator

Expression

Mammalian

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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TU#867 (Punc-119sid-1)

Plasmid

#25811

Depositor

Martin Chalfie

Article

Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

Insert

systemic RNA interference-deficient 1 (sid-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pASTA3

Plasmid

#24657

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

tdTomato

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pemiRFP703-N1

Plasmid

#136558

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-WPRE

Plasmid

#130994

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TyrRS-His

Plasmid

#124121

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tyrosyl-tRNA ligase (tyrS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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803 pGEX2T PTEN

Plasmid

#10784

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Article

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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optGluCl alpha EYFP

Plasmid

#15104

Depositor

Henry Lester

Article

Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

Insert

GluCl alpha (glc-1 Nematode)

Tags

EYFP

Expression

Mammalian

Mutation

Codon optimized cDNA

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pET_RTX(exo-)-6xHis

Plasmid

#145028

Purpose

pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

Depositor

Andrew Ellington

Article

Bhadra et al BioProtocol 2021

Insert

RTX(exo-)

Tags

6xHis

Expression

Bacterial

Mutation

N210D

Promoter

T7

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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