We narrowed to 8,394 results for: Dos;
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Plasmid#105773PurposeExpression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmCerulean-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only
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pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His
Plasmid#83458PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)DepositorInsertPolycystin-1 (PKD1 Human)
Tags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303, S4144DAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,S166A/pET28b(+)
Plasmid#83301Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Serine 166 …Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,T151A/pET28b(+)
Plasmid#83300Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Threonine 1…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,Y197F/pET28b(+)
Plasmid#83299Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197FDepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Tyrosine 19…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,E165A/pET28b(+)
Plasmid#83298Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Glutamic ac…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279A
Plasmid#60705Purposeretroviral expression of rat Cx43 S279A mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-S279/282A
Plasmid#60712Purposemammalian expression of rat Cx43 S279/282A mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-S279/282D
Plasmid#60714Purposemammalian expression of rat Cx43 S279/282D mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C
Plasmid#60394PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptideDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dFN
Plasmid#51842PurposeFlag-CHES1L mutant lacking the forkhead binding site and N-terminal domainsDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dC
Plasmid#51839PurposeFlag-CHES1L mutant lacking the C-terminal and NLS domainsDepositorAvailable SinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dN
Plasmid#51843PurposeFlag-CHES1L mutant lacking the N-terminal domainDepositorAvailable SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-DBD-NLS
Plasmid#51841PurposeFlag-CHES1L mutant encoding the DNA binding and NLS domains onlyDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSMutationcontains CHES1 amino acids 110-205Available SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dC-NLS
Plasmid#51840PurposeFlag-CHES1L mutant lacking the C-terminal, with remaining NLSDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSMutationcontains CHES1 amino acids 1-205Available SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1LdFC-NLS
Plasmid#51838PurposeFlag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLSDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSMutationcontains CHES1 amino acids 1-109Available SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES11L-dFC
Plasmid#51837PurposeFlag-CHES1L mutant lacking the forkhead binding site and the C-terminal domainsDepositorAvailable SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta-ATG1-mMCL1
Plasmid#45823PurposeThis plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.DepositorInsertDelta ATG1-mMCL1
ExpressionMammalianMutationThe first amino acid (ATG) is deleted from the fu…Available SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT
Plasmid#111072PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 BD (522-645, C643A)/pET28HMT
Plasmid#111073PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 BD (522-645, C643A)PromoterT7AvailabilityAcademic Institutions and Nonprofits only