We narrowed to 17,102 results for: EMB

1. pEAK10-His-Myc-Kal9

   Plasmid

   #25441

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal9
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pEAK10-His-Myc-Kal5

   Plasmid

   #25440

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal5
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

3. qTAG-C-dTAG-Zeo

   Plasmid

   #207738

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRSETB/mStayGold(c4)

   Plasmid

   #212018

   Purpose

   For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold(c4)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lact-C2-GFP

   Plasmid

   #22852

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 Domain (MFGE8 Bovine)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-dTAG-Puro

   Plasmid

   #207727

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Human NCKX6-FlagTag (pcDNA3.1+)

   Plasmid

   #75217

   Purpose

   Mammalian expression of SLC8B1

   Depositor

   Jonathan Lytton

   Article

   Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

   Insert

   SLC8B1 (SLC8B1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIBA2-Int-SpyTag

   Plasmid

   #198038

   Purpose

   Expression plasmid for producing SpyTagged stalled secretion variant of intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   lacks native signal peptide (amino acids 1-39)

   Promoter

   tet

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOGG202

   Plasmid

   #115503

   Purpose

   Clone of a level 1 unit which encodes IPTG-inducible sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Puro-dTAG

    Plasmid

    #207694

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. qTAG-N-Blast-mNeon

    Plasmid

    #207676

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Lyn-AKAR4

    Plasmid

    #61620

    Purpose

    Lipid raft-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    Lyn-AKAR4
    

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. EasyFusion T2A-H2B-mCherry

    Plasmid

    #113089

    Purpose

    General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-mCherry
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EasyFusion T2A-H2B-GFP

    Plasmid

    #112851

    Purpose

    General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-GFP
    

    Use

    Synthetic Biology

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSems LifeAct-StayGold

    Plasmid

    #222947

    Purpose

    Expression of LifeAct tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    LifeAct
    

    Tags

    StayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYFJ84

    Plasmid

    #227644

    Purpose

    Vibrio harveyi acyl-ACP synthetase

    Depositor

    John Cronan

    Article

    Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

    Insert

    Acyl-Acyl carrier protein synthetase
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. LV-Cre pLKO.1

    Plasmid

    #25997

    Purpose

    Lentiviral expression of nls-Cre from the CMV promoter

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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