We narrowed to 6,639 results for: itch
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Plasmid#231567PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOSV00157
Plasmid#222937PurposeKill switch plasmid for DNA sensor construction using Bacillus subtilisDepositorInsertTxpA-RatA toxin-antitoxin
ExpressionBacterialPromoterPhyperspankAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
Plasmid#219653PurposeGeneral mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark VenusDepositorInsert2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
UseAAVTags2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)ExpressionMammalianPromoternEf Addgene # 149296Available SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-LOVPLD-mCherry-OSBP(PH)
Plasmid#219561PurposeGolgi-targeted LOVPLDDepositorInsertLOVPLD-mCherry-OSBP(PH)
TagsmCherryExpressionMammalianAvailable SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-AcrAB-AsLOV2*(543)
Plasmid#210863PurposeIPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.DepositorInsertsacrA
acrB
UseSynthetic BiologyTagsAsLOV2*(543)Available SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-BMPR2a
Plasmid#207616PurposeZebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOVDepositorInsertBMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Vaucheria frigida, Zebrafish)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1T/pUBQ10(mTurq->mScarlet)
Plasmid#195947PurposeTp901 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Tp901-pUBQ10-attB_Tp901-mTurq-tUBQ10
ExpressionPlantAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22U6CatalE4PCh(#767)
Plasmid#184078Purposecyclofen-inducible Catalase activation in zebrafish permanent transgenicDepositorInsertCatalDeltaC-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes) + in vitro tra…MutationCatalDeltaC: deleted 516-527; ERT2: G400V, M543A,…PromoterZf-Ubi+SP6Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNUT2
Plasmid#175745PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 2DepositorInsertmCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
TagsmCherryExpressionMammalianAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En25EE4P3Che(#1213)
Plasmid#184099Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(5E)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …Promoter5xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2SE4P3Che(#1209)
Plasmid#184097Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22cUm5En2SE4P3Che(#1189)
Plasmid#184095Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22cUm5En2E4P3Che(#1188)
Plasmid#184094Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2E4P3Che(#1208)
Plasmid#184096Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …Promoter5xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBAR-Cry2PHR
Plasmid#176604PurposeCry2PHR with N-terminal IBAR domainDepositorInsertIBAR-Cry2PHR-mCherry
ExpressionMammalianPromoterCMVAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIS8-3_DL121
Plasmid#171953PurposeExpression vector for ecDHFR-asLOV2 fusion protein. Also has TYMSDepositorInsertFusion of ecDHFR and asLOV2 at position 121 of DHFR
Tags8-His tagExpressionBacterialPromoterT7Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_DL121_WTTS
Plasmid#171954PurposeSelection vector for chimeric fusion of ecDHFR and asLOV2.DepositorInsertsFusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase
Tags8-His tagExpressionBacterialPromoterT7Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
Plasmid#165029PurposeExpress cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherryDepositorInsertLaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
TagsmCherryExpressionBacterial and MammalianPromoterT7Available SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)
Plasmid#165026PurposeExpress cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
TagsGFPExpressionBacterial and MammalianPromoterT7Available SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only