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Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
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pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
UseTagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a/b-actin
Plasmid#82583Purpose5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionDepositorInsertEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-Gal80ts
Plasmid#51619Purposevertebrate expression of full-length temperature-sensitive Gal80 with stopDepositorInsertGal80ts (GAL80 Budding Yeast)
UseVertebrate expressionTagsExpressionMutationtemperature sensitive (G203V, G323R)*PromoterAvailable sinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_sgRNA_expression_in_pBluescript
Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorInsertSpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B(T116C)
Plasmid#157808PurposeExpression of human histone H2B with T116C mutation to facilitate maleimide chemistryDepositorInsertH2B(T116C)
UseTagsExpressionBacterialMutationT116CPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-smyhc1
Plasmid#126748PurposeZebrafish slow myosin heavy chain promoter. Clone 667DepositorInsertsmyhc1 (smyhc1 Zebrafish)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NICD1
Plasmid#46048DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseEntry vectorTagsMyc tagExpressionMutationCytoplasmic domain initiating at amino acid 1753PromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E_unc503
Plasmid#64020Purpose5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45bDepositorInsertmuscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ152
Plasmid#69303PurposeGateway entry vector with pco-dCas9-VP64DepositorInsertpco-dCas9-VP64 (Plant codon-optimized)
UseCRISPRTagsExpressionMutationVP64 activator fusion; NLS fusionPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
NmCas9_sgRNA_expression_in_pBluescript
Plasmid#122091PurposeU6 driven NmCas9 sgRNA expression vector for cloning own guidesDepositorInsertNmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R377
Plasmid#138247PurposeExpresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW610 - GR-LhG4_BD
Plasmid#115992Purposeentry vector for GreenGate cloning method, CDS, with adaptors for B and D modulesDepositorInsertGR-LhG4_BD
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only