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pGBW-m4134196

Plasmid

#152506

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BDH2_FL_BioN

Plasmid

#195870

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BDH2 (BDH2 Human)

Use

Pfbd-bira, baculovirus expression

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Promoter

Polyhedrin

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133280

Plasmid

#152958

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p8203 MSCV-neo C-V5 16E7

Plasmid

#193976

Purpose

HPV16 E7 expression, C-terminal V5 tag

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

HPV16 E7

Use

Retroviral

Tags

V5

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134342

Plasmid

#152111

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-CTSS

Plasmid

#158445

Purpose

Gateway Cloning compatible entry vector for the human CTSS gene.

Depositor

Alejandro Chavez, Sho Iketani

Insert

CTSS (CTSS Human)

Use

Gateway Cloning

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134407

Plasmid

#152787

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133478

Plasmid

#152804

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133907

Plasmid

#152492

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
ACSM2B

Plasmid

#39167

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ACSM2B (ACSM2B Human)

Use

Baculovirus expression

Tags

His Tev

Mutation

Actually the mitochondrial form, GI:58082049, wit…

Promoter

polyhedrin

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
IDG_CHRNB1_OE_1

Plasmid

#161659

Purpose

Overexpresses CHRNB1

Depositor

Mike McManus

Insert

CHRNB1 (CHRNB1 Human)

Tags

V5

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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IDG_KCNH8_OE_1

Plasmid

#161702

Purpose

Overexpresses KCNH8

Depositor

Mike McManus

Insert

KCNH8 (KCNH8 Human)

Tags

V5

Expression

Mammalian

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046672

Plasmid

#153918

Purpose

Yeast expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133362

Plasmid

#152447

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133802

Plasmid

#152925

Purpose

Mammalian expression plasmid for SARS-CoV-2 endoRNAse

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4134121

Plasmid

#152271

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133344

Plasmid

#152152

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133982

Plasmid

#152393

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133457

Plasmid

#151991

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp11

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pB-actin 18E6

Plasmid

#37434

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 18E6 (E6 HPV)

Expression

Mammalian

Promoter

Human B-actin

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only