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pFUW-TetO-NMI

Plasmid

#178446

Purpose

doxycycline-inducible expression of Human NMI in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

NMI (NMI Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro XLone-CCND2

Plasmid

#179845

Purpose

donor plasmid for inducible expression of CCND2 in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Expression

Mammalian

Promoter

TRE3GS

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Eomes

Plasmid

#70271

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Eomes

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Eomes (Eomes Mouse)

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Cdx2

Plasmid

#70697

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Cdx2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

Plasmid

#216475

Purpose

lentiviral dual expression of YAP5SA and eGFP

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
eGFP

Use

Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Gata3

Plasmid

#70270

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Gata3

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

GATA binding protein 3 (Gata3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ascl2

Plasmid

#71151

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ascl2/Mash2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ascl2 (Ascl2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Elf5

Plasmid

#70772

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Elf5

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Elf5 (Elf5 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ets2

Plasmid

#70272

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ets2 (Ets2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

Plasmid

#216477

Purpose

lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Cre/Lox and Lentiviral

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Hes1

Plasmid

#61534

Purpose

Expresses Hes1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes1 (Hes1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Lin28

Plasmid

#60345

Purpose

Mammalian Expression, Lentiviral

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Lin28 (Lin28a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-hs-CDC34

Plasmid

#52731

Purpose

retroviral expression of human CDC34

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

CDC34 (CDC34 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Id2

Plasmid

#70764

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

inhibitor of DNA binding 2 (Id2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
p2702-AGER-gRNA1

Plasmid

#216471

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2703-AGER-gRNA2

Plasmid

#216472

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2704-AGER-gRNA3

Plasmid

#216473

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2705-AGER-eGFP

Plasmid

#216474

Purpose

donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

eGFP

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-Hygro-2A-TMRPSS2

Plasmid

#170390

Purpose

Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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