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pLenti-gfaABC1D-monSTIM1

Plasmid

#161797

Purpose

Lentiviral vector expressing monSTIM1 in astrocytes.

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

gfaABC1D

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CamKIIa-monSTIM1

Plasmid

#161796

Purpose

Lentiviral vector expressing monSTIM1 in neurons.

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CamKIIa

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3- SREBP-1 (A317-318; L340)

Plasmid

#204195

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318; Gly to Leu 340

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3- SREBP-1 (A317-318;A341; A1171)

Plasmid

#204196

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341,1171

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-cka

Plasmid

#195067

Purpose

Expresses FLAG-tagged Drosophila cka protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Connector of kinase to AP-1 (Cka Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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CHMP2B_Halo_C_allele

Plasmid

#178153

Purpose

Donor vector for endogenous tagging of human CHMP2B at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (CHMP2B Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Ursula Rescher, Volker Gerke

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2

Plasmid

#73538

Purpose

Inducible lentiviral expression of Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L

Plasmid

#89610

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L (DOT1L Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)

Plasmid

#204199

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

3xHA, eGFP and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341,1171

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatQ61R

Plasmid

#206843

Purpose

To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Glutamine 61 to Arginine

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2181

Plasmid

#158421

Purpose

AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple32 (CLDN5 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple32

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2230

Plasmid

#158420

Purpose

AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple253 (PITX3 )

Use

AAV

Tags

Expression

Mutation

Promoter

Ple253

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2_RNAmut

Plasmid

#73539

Purpose

Inducible lentiviral expression of Ago2_RNAmut

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Small RNA binding mutant PAZ domain Y311A and F31…

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2_CATmut

Plasmid

#73540

Purpose

Inducible lentiviral expression of Ago2_CATmut

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Catalytic mutant PIWI domain D670A

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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LADL Bridge + Target Zfp462-Klf4SE

Plasmid

#127668

Purpose

Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a and hU6

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEQ111m

Plasmid

#40190

Depositor

Paul Modrich

Article

Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

Insert

EcoRI endonuclease & EcoRI methylase

Use

Tags

Expression

Bacterial

Mutation

changed Glutamic Acid 111 to Glutamine

Promoter

Lambda PL

Available since

Nov. 19, 2012

Availability

Academic Institutions and Nonprofits only