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Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1
Plasmid#138461Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64DepositorInsertMCP-VP64-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEH373 dTomato 3x high affinity MS2 loops
Plasmid#168221PurposeMammalian expression of dTomato with 3 high affinity MS2 loopsDepositorInsertdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-dCas9-VP64-MS2-VP64-ZsG
Plasmid#192669PurposeDox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1DepositorInsertdCas9-VP64, MS2-VP64, ZsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterTRE3GAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2
Plasmid#205160Purposeexpress β-ACTIN-3′UTR-8xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
Plasmid#205161Purposeexpress β-ACTIN-3′UTR-24xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry
Plasmid#205269PurposeHuman expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)DepositorInsertSpuFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-PABPC1 MLLEmut M161A/D165K
Plasmid#65810Purposeexpression of PABPC1-MS2 fusion, double mutant.DepositorInsertPABPC1 (PABPC4 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationMLLEmut M161A/D165KPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC
Plasmid#148443PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-ACG site)
Plasmid#170155PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editingDepositorInsertBoxB-RAB7A(ACG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-UAG site)
Plasmid#170138PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcriptDepositorInsertBoxB-RAB7A(UAG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro
Plasmid#192681PurposeLentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcriptionDepositorInsertMouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-Tev-MS2-p65-HSF1
Plasmid#84513PurposeMESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1DepositorInsertsV2-MESA-35F-Tev
MS2-P65-HSF1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
NG1301 pCI-TPI-4MS2-SMG5(short)-4H
Plasmid#65806PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4 MS2 stem loops, SMG5 3'UTR shortened versi…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA
Plasmid#222404Purposearabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.DepositorInsert5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
UseTags1xMS2 hairpin, 7GC clamp, TtrpA terminatorExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSS2 - pBait-1xMS2-7GC[empty]-TtrpA
Plasmid#222406Purposearabinose-induced synthesis of an RNA containing an MS2 hairpin, 7GC clamp and trpA terminator. Used as a three-hybrid control. For cloning purposes, see plasmid #222404.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-GAC site)
Plasmid#170149PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcriptDepositorInsertBoxB-RAB7A(GAC)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only