We narrowed to 1,229 results for: SUMO

1. pcDNA5-FRT/T0-3XFLAG-NFATC2IP SLD2

   Plasmid

   #221388

   Purpose

   Mammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 2 (SLD2, aa 345-418)

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Amino acids 1-344 of NFATC2IP deleted

   Promoter

   CMV

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA5-FRT/T0-eGFP-NFATC2IP SLD2

   Plasmid

   #221438

   Purpose

   Mammalian expression vector of eGFP tagged NFATC2IP SUMO-like domain 2 (SLD2, aa 345-418)

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Amino acids 1-344 of NFATC2IP deleted

   Promoter

   CMV

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. 371-S

   Plasmid

   #97034

   Purpose

   His6-Sumo-tev-yORF

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   His6 tag, SUMO domain, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSA1

   Plasmid

   #52261

   Purpose

   Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

   Plasmid

   #208039

   Purpose

   Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa; 3MAS) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, N-terminal Split-TurboID

   Expression

   Mutation

   3MAS mutant: K65R, K160R and K490R; mutated SIM

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SENP1 (catalytic domain)

   Plasmid

   #16356

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP1 catalytic domain (SENP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 419 to 644 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-SENP2 (catalytic domain)

   Plasmid

   #16357

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP2 catalytic domain (SENP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 365 to 590 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC-PreE2

   Plasmid

   #52264

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC-IntE2

   Plasmid

   #52265

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Synthetic, Human)
   

   Use

   Tags

   GST and GyrA-intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-SENP8 (full length))

    Plasmid

    #16361

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP8 full length (SENP8 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    human SENP8 full length, see Depositor Comments

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pThuAsol

    Plasmid

    #171675

    Purpose

    pThuAsol expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    SUMO-TEV-ThuAsol
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

    Plasmid

    #228646

    Purpose

    Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    MERS-Cov Mpro domain
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. CTA2-mNG211

    Plasmid

    #141167

    Purpose

    Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    SUMO-CTA2-mNG211
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 GATA1 K137R

    Plasmid

    #85694

    Purpose

    Mammalian expression of mouse GATA1 with K137R mutation

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K137R (SUMO-1 attachment site)

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

15. 6xHis-TEV-SMO-1(GG)

    Plasmid

    #87054

    Purpose

    Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    smo-1 (smo-1 Nematode)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    The last aa was removed to render processed SMO-1…

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid

    #208037

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    Ubnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5H9M (SIAH2)

    Plasmid

    #79521

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SIAH2 (SIAH2 Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

18. Human Coxsackievirus A16 strain G10 inactive 2A protease

    Plasmid

    #228665

    Purpose

    Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus A71 2A protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Avitag insertion and C2A mutations / Active site …

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. WNV isolate WN NY 2000-crow3356 NS5 RdRp full length

    Plasmid

    #228662

    Purpose

    Bacterial expression of the West Nile virus NS5 RdRp protein for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    West nile NS5 RdRp
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pET28a-SENP6 (catalytic domain)

    Plasmid

    #16359

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP6 catalytic domain (SENP6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 628 to 1112 catalytic domain of human …

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only