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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138132

Purpose

Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-mouse-IgG(kappa)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138128

Purpose

Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-mouse-IgG(kappa)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Plasmid

#125235

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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Human NANOS3-P2A-mCherry knock-in targeting construct

Plasmid

#59721

Purpose

Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

P2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Plasmid

#125225

Purpose

Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125231

Purpose

For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA

Plasmid

#125233

Purpose

Expresses "sender" trans-cellular NanoLuc in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-F152E-V5His6_AC

Plasmid

#148467

Purpose

Insect Expression of DmNanos_116-163-F152E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-F152E (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-L127DF130D-V5His6_AC

Plasmid

#148468

Purpose

Insect Expression of DmNanos_116-163-L127DF130D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-L127DF130D (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1

Plasmid

#183399

Purpose

Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-NanoLuc-FKF1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC

Plasmid

#148466

Purpose

Insect Expression of DmNanos_116-163-E151AF152AN1555A

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-E151AF152AN1555A (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

Plasmid

#175554

Purpose

Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

NANOG

Use

Mouse Targeting

Tags

FKBPV-HA2-P2A-eGFP

Expression

Mammalian

Mutation

none

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Use

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

Plasmid

#174643

Purpose

Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

Use

Tags

SSPB(nano)-GFP

Expression

Mammalian

Mutation

EGFP: Met1Del; ppKin14VIb(aa861-1321)

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)

Plasmid

#125224

Purpose

Expresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125236

Purpose

Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Plasmid

#125230

Purpose

For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Use

Lentiviral

Tags

HA,Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Plasmid

#125229

Purpose

Expresses transmembrane intracellular NanoLuc in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge