We narrowed to 5,093 results for: puc

1. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19 PKC delta

   Plasmid

   #8427

   Depositor

   Frederic Mushinski

   Article

   Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

   Insert

   Mouse Protein Kinase C delta (Prkcd Mouse)
   

   Expression

   Bacterial

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-rm_gCarvi-T2A

   Plasmid

   #190334

   Purpose

   Ratiometric reporter for cAMP

   Depositor

   Naoto Saitoh

   Article

   Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   ratiometric gCarvi
   

   Use

   Unspecified

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-hrDNA-12.0

   Plasmid

   #206320

   Purpose

   A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

   Depositor

   Brian McStay

   Article

   van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

   Insert

   Intergenic spacer of human rDNA repeat
   

   Use

   Cloning

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19 PKC alpha

   Plasmid

   #8429

   Depositor

   Frederic Mushinski

   Insert

   Mouse Protein Kinase C alpha (Prkca Mouse)
   

   Expression

   Bacterial

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pUC18/promEco/Lysostaphin

   Plasmid

   #125765

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK-Sa1 promoter

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-Amp_CRISPIE donor B5

   Plasmid

   #172846

   Purpose

   CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19/NLS-mChe

   Plasmid

   #183162

   Purpose

   Construct for transient expression of cell nucleus-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   NLS-mCherry
   

   Tags

   16 aa nuclear localization signal and 16 aa nucl…

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-AdEvolve-DEST

   Plasmid

   #131692

   Purpose

   A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Type

   Empty backbone

   Use

   Adenoviral

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-Amp_CRISPIE donor B1

    Plasmid

    #172842

    Purpose

    CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mEGFP
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-sgRNA-MS2-U6

    Plasmid

    #171694

    Purpose

    To construct expression vector for tBE-V5-mA3

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    sgRNA scaffold-MS2-U6
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19/mChe-Golgi

    Plasmid

    #183169

    Purpose

    Construct for transient expression of Golgi apparatus-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Golgi
    

    Tags

    AtRER1B, A. thaliana endoplasmic reticulum retrie…

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18-mini-Tn7T

    Plasmid

    #64954

    Purpose

    mini-Tn7 base vector with transcriptional terminator

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pUC18-mini-Tn7T-Gm-GW

    Plasmid

    #64961

    Purpose

    mini-Tn7 Gateway destination vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pUC18T-mini-Tn7T-Tp

    Plasmid

    #65024

    Purpose

    TpR mini-Tn7 vector, mobilizable

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Available Since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUCmini-iCAP-PHP.B3

    Plasmid

    #103004

    Purpose

    non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B3 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCmini-iCAP-PHP.B2

    Plasmid

    #103003

    Purpose

    non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-P70a-3WJdB-T

    Plasmid

    #87311

    Purpose

    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Three-way Junction dimeric Broccoli aptamer
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19/Mito-mChe

    Plasmid

    #183167

    Purpose

    Construct for transient expression of mitochondrion-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Mito-mCherry
    

    Tags

    OsCOX11, Oryza sativa cytochrome c oxidase assemb…

    Expression

    Plant

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCIDT-AMP-Bv16SrRNA

    Plasmid

    #218518

    Purpose

    pUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantification

    Depositor

    Ami Bhatt

    Article

    Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

    Insert

    16S rRNA
    

    Use

    Synthetic Biology

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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