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Plasmid#155306PurposeRfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFPDepositorInsertRfx Cas13d CRISPR RNA BFP
UseTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T
Plasmid#64957Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-T7-J1~398-Luc
Plasmid#208639Purposefor in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19DepositorInsertHepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
UseLuciferase; In vitro transcription templateTagsFirefly Luciferase (F-Luc and T7 promoterExpressionMutationnt 1~398, in frame with donstream F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7
Plasmid#64953Purposemini-Tn7 base vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-U6-terminator (SL695)
Plasmid#133961PurposepUC19 plasmid with U6 promoter followed by a poly-T termination signalDepositorInsertU6 promoter
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC118-FLIP-Puro
Plasmid#84538Purposea mock vector for generating FLIP targeting vectorsDepositorInsertFLIP - Puro
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.V2
Plasmid#127848Purposenon-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.V2 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B4
Plasmid#127849Purposenon-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B4 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-AMP-Bv16SrRNA
Plasmid#218518PurposepUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantificationDepositorInsert16S rRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19_dTAG-KANSL3-targeting
Plasmid#170755PurposeDonor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locusDepositorInsertBSD-P2A-2xHA-dTAG
UseMouse TargetingTagsBSD-P2A-2xHA-dTAGExpressionMutationPhenylalanine 36 to valine in FKBP12PromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IMBB2.4-pUC57-mini
Plasmid#88845Purposeplasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)DepositorTypeEmpty backboneUseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-white[coffee]
Plasmid#84006PurposeDonor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4DepositorInsertA 2,080 bp fragment of the white[coffee] allele (w Fly)
UseUnspecifiedTagsExpressionMutationA GC-to-AA mutation that creates a G589E missense…PromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57_beta-globin-5'UTR_Nanoluc
Plasmid#219989Purposebetaglobin-5'UTR-NanoLuc mRNA synthesisDepositorInsertbetaglobin-5'UTR-NanoLuc (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in LT)
Plasmid#70033PurposepUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding regionDepositorInsertTSPyV full genome, linearized in Large T-antigen
UseCloning vectorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pT7-eGFP
Plasmid#32552DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterpT7Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-3WJdB-T
Plasmid#87311PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.DepositorInsertThree-way Junction dimeric Broccoli aptamer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-YAP-mEGFP
Plasmid#166472PurposeHDR template for tagging of endogenous mouse YAP N-terminus with mEGFPDepositorInsertYAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B4-Leum
Plasmid#44312DepositorInsertUAS1B4-Leum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B4-LeumAvailable sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits