We narrowed to 13,726 results for: cas9

1. pG3B-U6EC1

   Plasmid

   #134756

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA-U6, zCas9-EC1

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG3H-U3Ub

   Plasmid

   #134749

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA- OsU3, zCas9-Ubi1

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-BtH

   Plasmid

   #133392

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6Rosa-CAG-Gal4-Rad18UBD

   Plasmid

   #119012

   Purpose

   Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG3GB411

   Plasmid

   #134748

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA- OsU3, zCas9-Ubi1

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-EAMP

   Plasmid

   #138265

   Purpose

   mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   iCas9 target-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-HTLV

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWp9-3

   Plasmid

   #73854

   Purpose

   pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Insert

   UBCp-RFP-CMVp-GFP-U6p-RFP-sg2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

8. PABE-5

   Plasmid

   #115626

   Purpose

   Targeted A to G in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   NLS-nCas9-NLS-wtTadA-TadA7.10-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CP1300-CBEmax

   Plasmid

   #119805

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   rApobec1-nSpCas9 (CP1300) -UGI-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-R4R3-stop

    Plasmid

    #113745

    Purpose

    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    stop cassette
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Added 3 stop codons to the original sequence; see…

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAct:MCP-VP64

    Plasmid

    #78904

    Purpose

    Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2:VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    March 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAWp9-1

    Plasmid

    #73852

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-GFP-sg1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET28(a)-p2c-eGFP

    Plasmid

    #242758

    Purpose

    Expresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coli

    Depositor

    Jason Rasgon

    Article

    Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

    Insert

    P2C-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMR-A-3-Sasg

    Plasmid

    #214349

    Purpose

    Expresses T7-RNAP, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    T7-RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPEY-Z3-Kan

    Plasmid

    #232102

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPEY-Z3-Hyg

    Plasmid

    #232101

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_S34F_U2AF1

    Plasmid

    #176592

    Purpose

    Adenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1

    Depositor

    Manoj Pillai

    Article

    Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1Cas-LT1

    Plasmid

    #210556

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAf-CRISPR-ctfR1

    Plasmid

    #207992

    Purpose

    CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    ctfR1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Modular BE4max for GG insertion

    Plasmid

    #198891

    Purpose

    Easily insert deaminase of choice into BE4max using Golden Gate cloning

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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