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Showing: 5621 - 5640 of 19449 results

  7. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid
    #157732
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nprot-HF_GC3opt (N SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available since
    Aug. 10, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157732-plasmid-map-sequence-id-308146

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  8. pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS

    Plasmid
    #123200
    Purpose
    binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Publication
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
    Use
    Luciferase
    Expression
    Plant
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123200-plasmid-map-sequence-id-242251

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  9. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

    Plasmid
    #111924
    Purpose
    Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ
    Depositor
    Takeharu Nagai
    Publication
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OeNL(Ca2+)_18μ
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111924-plasmid-map-sequence-id-214007

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  10. mEos3-caRac1

    Plasmid
    #92041
    Purpose
    Expresses mEos3 fusion with caRac1 (Q61L)
    Depositor
    Ji Yu
    Publication
    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAC1 (RAC1 Human)
    Tags
    mEos3
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Q61L (constitutively active)
    Promoter
    CMV
    Available since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92041-plasmid-map-sequence-id-180751

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  11. EGFP-Caveolin3DGV

    Plasmid
    #67886
    Purpose
    Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells
    Depositor
    Rob Parton
    Publication
    Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cav3 (Cav3 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of the first 53 amino acids of Caveolin3
    Promoter
    CMV
    Available since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. PKA-N-HiBiT

    Plasmid
    #211377
    Purpose
    Bacterially expressed HiBiT-PKA for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with N-terminal HiBiT (PRKACA Human)
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    211377-plasmid-map-sequence-id-417163

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  14. pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234447
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Publication
    Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234447-plasmid-map-sequence-id-465730

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  15. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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  18. pDIV537

    Plasmid
    #177704
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii
    Depositor
    Uffe Mortensen
    Publication
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177704-plasmid-map-sequence-id-352590

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  20. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid
    #123181
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Publication
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    Use
    Luciferase; Part for plant expression
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123181-plasmid-map-sequence-id-241286

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Showing: 5621 - 5640 of 19449 results