We narrowed to 14,476 results for: nts

1. pAAV-CAG-NLS-GFP

   Plasmid

   #104061

   Purpose

   AAV expression of nuclear localized GFP with SV40 NLS

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB

   Insert

   GFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpRY-ABE8e

   Plasmid

   #185671

   Purpose

   Expresses SpRY-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-nSpRY
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   SpRY D10A

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

   Plasmid

   #86424

   Purpose

   PCR tagging module with yeast codon optimized mTurquoise2

   Depositor

   Sue Jaspersen

   Article

   Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

   Insert

   mTurquoise2
   

   Expression

   Yeast

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_Pro-IL-1α

   Plasmid

   #214341

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1A (IL1A Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUltra

   Plasmid

   #24129

   Purpose

   3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

   Depositor

   Malcolm Moore

   Article

   Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET3a-hUBA1

   Plasmid

   #63571

   Purpose

   Expression of human UBA1 tagged with His

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBA1 (UBA1 Human)
   

   Expression

   Bacterial

   Mutation

   Isoform 2 (1018 amino acids)

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB440

   Plasmid

   #74826

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_PEmax-2A-BSD

   Plasmid

   #234071

   Purpose

   For lentiviral expression of PEmax with blasticidin resistance

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Insert

   PEmax
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF-1α core promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Promoter

    MBP

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ132B2.0

    Plasmid

    #99888

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB501

    Plasmid

    #74843

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAW240

    Plasmid

    #170791

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Expression

    Yeast

    Promoter

    10B2 (P1 specific promoter)

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE347

    Plasmid

    #153228

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T5/Lac

    Available Since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGWB454

    Plasmid

    #74836

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT/TO_H2B_HaloTag7

    Plasmid

    #169329

    Purpose

    Expression of H2B-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 (H2BC21 Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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