Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,184 results for: Pol;

Showing: 8461 - 8490 of 9184 results

  5. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105635-plasmid-map-sequence-id-203205

    Enlarge

  6. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid
    #105637
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105637-plasmid-map-sequence-id-203819

    Enlarge

  8. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

    Enlarge

  9. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175788
    Purpose
    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175788-plasmid-map-sequence-id-345285

    Enlarge

  11. EGFP–acVHH–PB

    Plasmid
    #169685
    Purpose
    An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody
    Tags
    modified STIM1-PB tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169685-plasmid-map-sequence-id-338140

    Enlarge

  12. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid
    #170316
    Purpose
    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.
    Depositor
    Andrew Carter
    Article
    Mali et al Science. 2021 Feb 26;371(6532):910-916.
    Insert
    Q22YU3 C-terminal truncation (Delta-C3)
    Tags
    Strep-Tag II
    Expression
    Insect
    Mutation
    Deletion of C-terminal C3-finger
    Promoter
    polyhedrin
    Available Since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    170316-plasmid-map-sequence-id-333816

    Enlarge

  14. pOCC17

    Plasmid
    #118875
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and tagRFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118875-plasmid-map-sequence-id-232352

    Enlarge

  15. pOCC16

    Plasmid
    #118870
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    Tags
    EGFP and HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118870-plasmid-map-sequence-id-232333

    Enlarge

  16. pOCC218

    Plasmid
    #118907
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS10, cleavable with 3C protease, YBBR tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118907-plasmid-map-sequence-id-232329

    Enlarge

  17. pOCC18

    Plasmid
    #118868
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, tagRFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118868-plasmid-map-sequence-id-232324

    Enlarge

  18. pOCC171

    Plasmid
    #118888
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; SNAP tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118888-plasmid-map-sequence-id-232245

    Enlarge

  19. pOCC202

    Plasmid
    #118880
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Tags
    mKate2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118880-plasmid-map-sequence-id-232243

    Enlarge

  20. pOCC139

    Plasmid
    #118872
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mGFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118872-plasmid-map-sequence-id-232236

    Enlarge

  29. JARID1BA-c539

    Plasmid
    #79643
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JARID1B (KDM5B Human)
    Use
    Baculovirus expression
    Tags
    6x His and TEV cleavage site
    Promoter
    polyhedrin
    Available Since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 8461 - 8490 of 9184 results