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PM-FRB-CFP

Plasmid

#67517

Purpose

FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
TfR-pHoran4

Plasmid

#61547

Purpose

Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

Depositor

David Perrais

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

Transferrin receptor (TFRC Human)

Tags

pHoran4

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCD86-EGFP

Plasmid

#133858

Purpose

Mammalian expression of CD86 C-terminally tagged with EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYCL_P2A_Hygro_Barcode

Plasmid

#120462

Purpose

Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCL (MYCL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcI-ts,ind+-(NSP8)

Plasmid

#160656

Purpose

Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Insert

SARS-CoV-2 NSP8 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TAK11

Plasmid

#227095

Purpose

Luciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vector

Depositor

Farah Mustafa

Article

Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

Insert

CMV Promoter and HIV-1 TAR region

Use

Luciferase

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-Nb37

Plasmid

#205515

Purpose

Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-Nb37

Tags

Nuclear localization signal (NLS) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hNPPC-RFP

Plasmid

#22921

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pHR-CAG_3C-Twin-Strep

Plasmid

#113901

Purpose

Mammalian protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CAG

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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NET31 mRFP-N2 (490)

Plasmid

#61984

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

Insert

NET31 (TMEM209 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PresentER-MSIIFFLPL (GFP)

Plasmid

#102946

Purpose

PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

MSIIFFLPL (Serpinf1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5 mSynCAM 2

Plasmid

#115497

Purpose

expression of mouse full-length SynCAM 2

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm2 (Cadm2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pChlamy/Cre

Plasmid

#205440

Purpose

An expression vector for Cre recombinase (codon usage for C. reinhardtii)

Depositor

Masamichi Kamihira

Article

Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

Insert

Cre recombinase gene

Use

Chlamydomonas expression

Promoter

Heat shock protein 70A (HSP70A)/ribulose bisphosp…

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-CD4-FLAG

Plasmid

#51604

Purpose

expresses CD4 with C-terminal FLAG tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4 (CD4 Human)

Tags

FLAG and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

Plasmid

#164041

Purpose

CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

mCherry-CRISPR-TagV2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGM202T7

Plasmid

#69993

Purpose

E. coli/Streptomyces expression vector containing the T7-promoter

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aphII
tsr
rep
sso
T7 promoter
dso
His-Orf

Tags

N-terminal and C-terminal His tag encoding sequen…

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-Halo

Plasmid

#195163

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pGEX2TK

Plasmid

#71379

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

Insert

Grp1-PH (Cyth3 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only