161,152 results

1. pCMV-TadA8e-Ns3h

   Plasmid

   #221227

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and Ns3h helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-Ns3h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUltra-Plus-H2B-EGFP

   Plasmid

   #161776

   Purpose

   3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Insert

   H2B-EGFP from Addgene Plasmid #26790
   

   Use

   Lentiviral; Bicistronic

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   UbC and UbC promoter

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-EGFP (BPK1098)

   Plasmid

   #133962

   Purpose

   CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription

   Depositor

   Benjamin Kleinstiver

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHen2 Avi-tag-linker-p3

   Plasmid

   #119819

   Purpose

   For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   linker with Avi-tag and p3
   

   Use

   Tags

   Avi-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGTag-NLS-eGFP-SV40

   Plasmid

   #117811

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3XFlagNICD3

   Plasmid

   #20185

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 3 Intracellular Domain (Notch3 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 3 Intracellular Domain (Met…

   Promoter

   CMV

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Promoter

   Available since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV-AR

   Plasmid

   #213819

   Purpose

   Lenti-viral expression of human androgen receptor wild-type (WT).

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR (human androgen receptor) (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag (N terminal on backbone)

   Expression

   Mammalian

   Mutation

   No, it is AR wild-type.

   Promoter

   EF-1a

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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