We narrowed to 58,555 results for: tra

1. Plas-gRNA-Z3EV

   Plasmid

   #157659

   Purpose

   GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   GFP-gRNA NatMX Z3EV
   

   Use

   Insert storage (replicative in e. coli)

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2xVlk1-3xFlag

   Plasmid

   #172773

   Purpose

   mRNA synthesis for injection into embryos

   Depositor

   Edward De Robertis

   Article

   Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

   Insert

   Vlk1 (pkdcc.1.S Frog)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AY42_pS.Donor.R5

   Plasmid

   #100291

   Purpose

   CCR5-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Expression

   Mammalian

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. SP6-hCas9-Ce-mRNA

   Plasmid

   #47911

   Purpose

   In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Insert

   hCas9 with C. elegans 5' and 3' UTRs
   

   Use

   CRISPR

   Promoter

   SP6

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSV(X) C/EBPdelta

   Plasmid

   #13394

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPdelta (Cebpd Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. p CIneo-RL-Let7-perf

   Plasmid

   #115367

   Purpose

   Expression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTR

   Depositor

   Witold Filipowicz, Ramesh Pillai

   Article

   Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAN147

   Plasmid

   #129202

   Purpose

   yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   SUP35

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ1272

   Plasmid

   #44486

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, MCS
   

   Use

   Multiple cloning site vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDrop

   Plasmid

   #184872

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pColE1_sgRNA_M1S_AmpR

    Plasmid

    #119153

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTetQCas-BsaI

    Plasmid

    #170629

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. YF-Rac1(DN)

    Plasmid

    #20151

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(DN) (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    dominant negative mutation

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCasTns(Vch)-entry(BsaI)

    Plasmid

    #190273

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
    

    Expression

    Bacterial

    Mutation

    no

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)

    Plasmid

    #107135

    Purpose

    cGAL-C driver under the control of C. elegans myo-2 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Expression

    Worm

    Promoter

    C. elegans myo-2 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. hSERT-N1-pECFP

    Plasmid

    #70106

    Purpose

    mammalian expression of hSERT with C-terminal CFP fusion

    Depositor

    Harald Sitte

    Article

    Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

    Insert

    SERT (SLC6A4 Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDOC-K-glmS

    Plasmid

    #158058

    Purpose

    Donor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Inserts

    glmS homologous region 1
    glmS homologous region 2 + MCS3
    

    Use

    Synthetic Biology

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ2359 - intron GG2 - 250bp

    Plasmid

    #159878

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG2 - 250bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV/EF1a-DIO-SCLM

    Plasmid

    #216759

    Purpose

    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    EF1a (human elongation factor 1 alpha)

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ1149 - intron 3 smu-2 PATCs

    Plasmid

    #161516

    Purpose

    PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron 3 from smu-2
    

    Expression

    Worm

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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