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DGUOK gRNA (BRDN0001147023)

Plasmid

#76602

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGUOK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGUOK (DGUOK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001148670)

Plasmid

#77564

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001146365)

Plasmid

#77565

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001144918)

Plasmid

#77566

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Stx3 triple mutant (F31A D33A E34A)

Plasmid

#86945

Purpose

Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)

Depositor

Thomas Weimbs

Article

Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. do

Insert

STX3 triple mutant (F31A D33A E34A) (STX3 Human)

Tags

myc-His6

Expression

Mammalian

Mutation

triple mutant (F31A D33A E34A)

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(100-263)

Plasmid

#72548

Purpose

expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3xFlag and SV40 NLS

Expression

Mammalian

Mutation

100-263 aa deletion

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP d344-873, K45E

Plasmid

#21735

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Available Since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100/125/147/213-siResist

Plasmid

#49858

Purpose

Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, Methionine 147, an…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
lentiTet-LDLR-mCherryFus-D221Y BlastR

Plasmid

#254325

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant D221Y introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

D221Y

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-S213L BlastR

Plasmid

#254322

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant S213L introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

S213L

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-K223E BlastR

Plasmid

#254324

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant K223E introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

K223E

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-G219D BlastR

Plasmid

#254323

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant G219D introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

G219D

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-E240K BlastR

Plasmid

#254332

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant E240K introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

E240K

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-D221G BlastR

Plasmid

#254333

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant D221G introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

D221G

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-D224H BlastR

Plasmid

#254326

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant D224H introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

D224H

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-E101K BlastR

Plasmid

#254331

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant E101K introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

E101K

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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lentiTet-LDLR-mCherryFus-D227E BlastR

Plasmid

#254334

Purpose

Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene, variant D227E introduced in LDLR

Depositor

Richard Sherwood

Article

Zhou et al bioRxiv [Preprint]. 2025 Dec 18:2025.12.

Insert

LDLR (LDLR Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

D227E

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-WTUBA7-IRES-mcherry

Plasmid

#246475

Purpose

Mammalian expression of AcGFP-tagged WT human UBA7, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience. 2026 Mar 30;29(5):115454. doi:

Insert

AcGFP-WTUBA7-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-UBA7-V548L-IRES-mcherry

Plasmid

#246476

Purpose

Mammalian expression of AcGFP-tagged human UBA7-V548L, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience. 2026 Mar 30;29(5):115454. doi:

Insert

AcGFP-UBA7-V548L-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

V548L

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-UBA7-K709Sfs-IRES-mcherry

Plasmid

#246477

Purpose

Mammalian expression of AcGFP-tagged human UBA7-K709Sfs, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience. 2026 Mar 30;29(5):115454. doi:

Insert

AcGFP-UBA7-K709S*fs-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

K709Sfs*

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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