We narrowed to 15,732 results for: LEA

1. pSOMA-NES

   Plasmid

   #118939

   Purpose

   Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPPEDs

   Plasmid

   #162469

   Purpose

   protoplast test vector of pPPED

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Prime editing destination vector for transient ex…

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV2-miniSOG-VAMP2-T2A-mCherry

   Plasmid

   #50970

   Purpose

   AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   miniSOG-VAMP2-T2A-mCherry (Vamp2 Mouse, Arabdopsis thaliana)
   

   Use

   AAV

   Tags

   T2A-mCherry and miniSOG

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. myristoylated ct-GRK2

   Plasmid

   #178734

   Purpose

   N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

   Depositor

   Ege Kavalali

   Article

   Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

   Insert

   GRK2 (GRK2 Bovine)
   

   Use

   Lentiviral

   Tags

   Src 1-15

   Mutation

   only amino acids 548-671

   Promoter

   Ubiquitin

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO-VMAT2-WPRE-pA

   Plasmid

   #39339

   Depositor

   Bernardo Sabatini

   Article

   Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV; R

   Mutation

   Myc tag inserted in the first lumenal loop betwee…

   Available Since

   Oct. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-Neuromodulin-IRES-Puromycin

   Plasmid

   #134666

   Purpose

   Expresses EGFP-tagged plasma membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Neuromodulin (GAP43 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-G2-ATP8-V5 (puromycin)

   Plasmid

   #86852

   Purpose

   mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP8 (ATP8 Human)
   

   Tags

   ATP5G2 (G2) mitochondrial targeting signal and V5

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKII-STChRger2-TS-EYFP

   Plasmid

   #129393

   Purpose

   Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   soma targeted ChRger2
   

   Use

   AAV

   Tags

   Kv2.1-TS-EYFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEZT-GST-SLFN2

   Plasmid

   #174319

   Purpose

   Expression of recombinant GST-SLFN2

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   GST-SLFN2 (Slfn2 Mouse)
   

   Use

   Bacmam, baculovirus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ad-Flag-XBP1u

    Plasmid

    #63677

    Purpose

    Adenoviral vector for XBP1u expression

    Depositor

    Lingfang Zeng, Qingbo Xu

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI syn iGluu

    Plasmid

    #106122

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-DIO-GRAB_Ado1.0med

    Plasmid

    #153292

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sniper-Cas9

    Plasmid

    #138559

    Purpose

    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    Sniper-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET26b-SET

    Plasmid

    #8822

    Depositor

    Judy Lieberman

    Article

    Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

    Insert

    SET (SET Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

17. AM77_pU6.Sa-gRNA.CLYBL

    Plasmid

    #199237

    Purpose

    Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. aureus gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNAintron-CHIKV-nsp3-c3xFLAG

    Plasmid

    #215696

    Purpose

    Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp3
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRevTRE MKL1-N100

    Plasmid

    #19848

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1-N100 (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    deleted amino acids 1-100

    Available Since

    Jan. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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