We narrowed to 9,812 results for: Met;
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Plasmid#207402PurposeExpresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.DepositorInsertFibroblast Activation Protein Alpha (FAP Human)
UseRetroviralMutationchanged Serine 624 to Alanine. It's a protea…Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Blast FAP A657S
Plasmid#207403PurposeExpresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.DepositorInsertFibroblast Activation Protein Alpha (FAP Human)
UseRetroviralMutationchanged Alanine 657 to Serine. It's an endop…Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)
Plasmid#214879PurposeLentiviral vector encoding RfxCas13d targeting SURF2 guide arrayDepositorInserthU6-crSURF2-EFS-PuroR-WPRE
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
35S::Cel1SP-GeNL/pCAMBIA1301
Plasmid#190069PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplastDepositorInsertCel1 signal peptide-Green-enhanced Nano-lantern
ExpressionPlantPromoterCaMV 35SAvailable SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-pEGFP-C1
Plasmid#177416PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2.DepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGREAT17
Plasmid#170905PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20_SLC38A2_N82A
Plasmid#156183PurposeDox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.DepositorInsertSLC38A2 (SLC38A2 Human)
ExpressionMammalianMutationSilent mutations to prevent targeting by sgRNA: T…PromoterTREAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-HA-UFL1-RAMP4
Plasmid#139863PurposeLentiviral expression of fusion of human UFL1 (full length) with RAMP4DepositorInsertUFL1-RAMP4 (UFL1 Human)
UseLentiviralTagsHAMutationrecombinant fusion of UFL1 (full length) with RAM…Available SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CLV1 L2_pECIA14
Plasmid#114900PurposePrey vector CLV1 L2_pECIA14 should be used with bait vector CLV1 L2_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK1 A6_pECIA14
Plasmid#114767PurposePrey vector SERK1 A6_pECIA14 should be used with bait vector SERK1 A6_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
BRI1 BRI1_pECIA2
Plasmid#115086PurposeBait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.DepositorInsertAT4G39400 (BRI1 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
RPK2 X042_pECIA2
Plasmid#115135PurposeBait vector RPK2 X042_pECIA2 should be used with prey vector RPK2 X042_pECIA14.DepositorInsertAT3G02130 (RPK2 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR2Q105y
Plasmid#101026Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
Tagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-Y117C-FL
Plasmid#110219PurposeExpression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsHis6 and ZBExpressionBacterialMutationY117C, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3F2
Plasmid#73428PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.DepositorInsertRepressor C4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-5F5-mCherry
Plasmid#73422PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.DepositorInsertPromoter 5F5 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP5F5 (orthogonal T7-lac variant)Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT mir-1-1 reporter cel-mir-40
Plasmid#46739DepositorInsertcel-mir-40
UseRNAiTagsHis and hsa-mir-1-1ExpressionInsectPromoterMetallothionein (MT)Available SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
TagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only