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We narrowed to 6,761 results for: SIM;

Showing: 5721 - 5740 of 6761 results

  1. pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

    Plasmid
    #114624
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P70C; 43 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114624-plasmid-map-sequence-id-222739

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  3. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

    Plasmid
    #114630
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 49 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114630-plasmid-map-sequence-id-222716

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  4. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

    Plasmid
    #114610
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114610-plasmid-map-sequence-id-222711

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  5. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

    Plasmid
    #114609
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P70C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114609-plasmid-map-sequence-id-222709

    Enlarge

  6. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

    Plasmid
    #114620
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 37 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114620-plasmid-map-sequence-id-222661

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  7. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

    Plasmid
    #114619
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P80C; 37 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114619-plasmid-map-sequence-id-222659

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  8. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

    Plasmid
    #114618
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, A60C; 37 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114618-plasmid-map-sequence-id-222655

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  9. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

    Plasmid
    #114608
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, A60C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114608-plasmid-map-sequence-id-222653

    Enlarge

  11. pLuc-Optop38i-dsm

    Plasmid
    #89748
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89748-plasmid-map-sequence-id-192493

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  17. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  19. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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  20. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

    Enlarge

Showing: 5721 - 5740 of 6761 results