We narrowed to 15,732 results for: LEA

1. EGFP-Ki-67(RASA)-mCherry_IRESpuro2

   Plasmid

   #182921

   Purpose

   Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

   Depositor

   Daniel Gerlich

   Article

   Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

   Insert

   MKI67 (MKI67 Human)
   

   Tags

   EGFP and mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   RASA mutation

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HOXB8 (murine) HIS-tag pET

   Plasmid

   #8540

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB8 (Hoxb8 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   starts at amino acid #2 (Ser)

   Available Since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pYPQ141-ZmUbi-RZ-Fn

   Plasmid

   #108864

   Purpose

   FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Type

   Empty backbone

   Use

   CRISPR; Gateway compatible crrna entry clone

   Expression

   Plant

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUB-nHyPer2 (NLS:HyPer2:NLS)

   Plasmid

   #84730

   Purpose

   Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEB-Cas9-VirD2 (PCO)

   Plasmid

   #139802

   Purpose

   Expressing Cas9-VirD fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   Cas9-VirD2
   

   Tags

   Cas9-VirD2

   Expression

   Plant

   Promoter

   Ubiquitin

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUDE1093

   Plasmid

   #204227

   Purpose

   Episomal yeast plasmid expressing the Ercas12a

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Insert

   Eubacterium rectale cas12a (Ercas12a)
   

   Expression

   Yeast

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SynI-CreOn-FlpOff-EGFP

   Plasmid

   #176274

   Purpose

   Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin I

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mADAM10 E385A in pQCXIP cFLAG

   Plasmid

   #19137

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM10 (Adam10 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E385A

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

   Plasmid

   #166411

   Purpose

   express hPOT1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   hPOT1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Promoter

   ef-1a

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

    Plasmid

    #127864

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pASPIre1

    Plasmid

    #154842

    Purpose

    Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

    Insert

    Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    

    Expression

    Bacterial

    Promoter

    Prha

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-LGC-53

    Plasmid

    #86399

    Purpose

    Mammalian expression of codon-optimized dopamine receptor LGC-53

    Depositor

    Steven Mennerick

    Article

    Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

    Insert

    LGC-53 (lgc-53 Nematode, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression; Signal …

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCL.175

    Plasmid

    #184996

    Purpose

    Express -Eco1 HEK3 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK3 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCL.177

    Plasmid

    #184998

    Purpose

    Express -Eco1 EMX1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    EMX1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDR348 FANCF

    Plasmid

    #47510

    Purpose

    human gRNA expression vector targeting FANCF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-FANCF (FANCF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. PRE-Hsp70BbCas9_1.2

    Plasmid

    #190796

    Purpose

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Expression

    Insect

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pASPIre3

    Plasmid

    #154844

    Purpose

    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

    Insert

    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP C3-YAP-deltaC

    Plasmid

    #21126

    Depositor

    Marius Sudol

    Article

    Tsutomu Oka & Marius et al

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    EGFP-C3

    Expression

    Bacterial and Mammalian

    Mutation

    5 Carboxy-terminal amino acids -FLTWL are deleted

    Available Since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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