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pMpGWBhis03-Citrine-NLS

Plasmid

#188558

Purpose

Binary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

Citrine-NLS and MpIGPDm

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAV2/9-shMBVR-HO1-Fd-Fnr-CW3SL

Plasmid

#184003

Purpose

AAV production

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

shMBVR-HO1-Fd-Fnr-CW3SL

Use

AAV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2At(2nd)

Plasmid

#183085

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2At(2nd)

Plasmid

#183086

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2At(2nd)

Plasmid

#183087

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DUX4_KBM pDonor221

Plasmid

#187802

Purpose

DUX4 with KBM (p. E414-L424 deletion) entry clone

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

DUX4-KBM (DUX4 Human)

Expression

Mammalian

Mutation

p. E414-L424 deletion

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pZA52

Plasmid

#174765

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V with STOP codon

Use

Unspecified

Mutation

L17E/D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA50

Plasmid

#174763

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V with STOP codon

Use

Unspecified

Mutation

D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA51

Plasmid

#174764

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E with STOP codon

Use

Unspecified

Mutation

L17E + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA49

Plasmid

#174762

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon

Use

Unspecified

Mutation

WT + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJH53_pE12_hyCal2

Plasmid

#160408

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 hyCal2

Use

Other

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH54_pE12_hyCal7

Plasmid

#160409

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 hyCal7

Use

Other

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH55_pE12_hyCal9

Plasmid

#160410

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 hyCal9

Use

Other

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH120_pE12_HyPep_emGFP

Plasmid

#160406

Purpose

Gateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySyn

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyPep emGFP

Use

Other

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH161_pE12_HyPep_mRuby

Plasmid

#160407

Purpose

Gateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySyn

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyPep mRuby

Use

Other

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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