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pCRIS-PITChv2-FBL

Plasmid

#63672

Purpose

PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

Depositor

Takashi Yamamoto

Publication

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP-2A-PuroR

Use

CRISPR

Promoter

Promoterless

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pGP-CMV-GCaMP6m

Plasmid

#40754

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Publication

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCaMP6m

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

62 Articles

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

67 Articles

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Publication

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Publication

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Publication

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Publication

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pML104

Plasmid

#67638

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

Depositor

John Wyrick

Publication

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52 and pTDH3

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pET21a-Streptavidin-Alive

Plasmid

#20860

Purpose

Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

Depositor

Alice Ting

Publication

Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Insert

Streptavidin-Alive

Tags

His

Expression

Bacterial

Mutation

Wt gene

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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