162,717 results

1. AAV-SYN-mBaoJin-oPRE

   Plasmid

   #218683

   Purpose

   AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

   Depositor

   Bart Nieuwenhuis

   Insert

   mBaoJin
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW2082

   Plasmid

   #236576

   Purpose

   ISYmu1 PDS3 gRNA12 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. ADRA2A-Tango

   Plasmid

   #66216

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRA2A (ADRA2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ179

   Plasmid

   #85996

   Purpose

   One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   EGFP-NT2_cr2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified mouse U6

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

   Plasmid

   #169233

   Purpose

   Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

   Depositor

   Dora Tang

   Article

   Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

   Insert

   6xHis mCherry F30-2xdBroccoli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEmax_2star_pet21A

   Plasmid

   #227675

   Purpose

   Expression plasmid for PEMAX** protein in bacteria.

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   PEmax_2_star
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. STING-V2

   Plasmid

   #124264

   Purpose

   C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

   Depositor

   Eric Schirmer

   Insert

   TMEM173 (STING1 Human)
   

   Use

   Tags

   Venus2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-dCas9-p300_Blast

   Plasmid

   #192653

   Purpose

   3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Parkin

   Plasmid

   #187897

   Purpose

   Mammalian cell expression of untagged Parkin

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   PRKN (PRKN Human)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304-VHL-IRES-GFP

    Plasmid

    #187641

    Purpose

    VHL Expression vector with GFP

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    VHL (VHL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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