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We narrowed to 12,497 results for: BASE

Showing: 1121 - 1160 of 12497 results

  1. pYPQAT35

    Plasmid
    #223407
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223407-plasmid-map-sequence-id-452579

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  2. pYPQAT36

    Plasmid
    #223408
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223408-plasmid-map-sequence-id-454249

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  3. pYPQ121-Split-TALED

    Plasmid
    #218171
    Purpose
    Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein
    Depositor
    Yiping Qi
    Article
    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.
    Insert
    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
    Use
    Plastid base editing
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    218171-plasmid-map-sequence-id-437197

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  5. pMM7

    Plasmid
    #201213
    Purpose
    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
    Depositor
    Alex Nielsen
    Article
    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1
    Inserts
    sfGFP
    Spo0A right flank
    Spo0A left flank
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    F11V, T13P, N39D, A179A
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201213-plasmid-map-sequence-id-405310

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  13. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

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  14. pcDNA3-mCherry-PKCsub-RLuc8N-NES

    Plasmid
    #138213
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKCsub-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138213-plasmid-map-sequence-id-307415

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  15. pQC V5 MCS IRES G418

    Plasmid
    #110345
    Purpose
    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110345-plasmid-map-sequence-id-213720

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  16. pQC V5 MCS IRES Puro

    Plasmid
    #110342
    Purpose
    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110342-plasmid-map-sequence-id-218292

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  17. pcDNA5/FRT/TO/Flag-tGFP

    Plasmid
    #110375
    Purpose
    Mammalian expression of turboGFP with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    turboGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110375-plasmid-map-sequence-id-213401

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  18. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

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  19. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

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  20. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  21. pSG1016-2xBsaI-SaKKH_P2A_EGFP

    Plasmid
    #239461
    Purpose
    Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.
    Depositor
    Alexis Komor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239461-plasmid-map-sequence-id-476832

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  22. pQdCas9-sggfp

    Plasmid
    #236184
    Purpose
    The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Use
    Tags
    Expression
    Mutation
    Promoter
    Quorum sensing promoter
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    236184-plasmid-map-sequence-id-470109

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  24. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    NFAT enhancer + synthetic TATA box
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

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  27. pOET5 rota-VLP

    Plasmid
    #218147
    Purpose
    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).
    Depositor
    Minna Hankaniemi
    Insert
    Rotavirus VP6
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218147-plasmid-map-sequence-id-433323

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  28. THINGL-RR pcDNA3.1

    Plasmid
    #230047
    Purpose
    Expressing the human version of mScarletI-T2A-TINGL biosensor for glucose (named THINGL-RR), ratiometric variant with mScarletI.
    Depositor
    Bas Teusink
    Article
    Botman et al bioRxiv 2024.11.29.626064
    Insert
    THINGL-RR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230047-plasmid-map-sequence-id-458390

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  30. pDGB3_alpha1_35S-eGFP-T35S (GB4279)

    Plasmid
    #225434
    Purpose
    Transcriptional unit for constitutive expression of the enhanced GFP.
    Depositor
    Diego Orzaez
    Insert
    35S:eGFP:T35S
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225434-plasmid-map-sequence-id-447741

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  38. epi-ABEmax-NG

    Plasmid
    #135976
    Purpose
    Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only

  39. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid
    #207154
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNA15 (GNA15 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position E(244)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207154-plasmid-map-sequence-id-410274

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Showing: 1121 - 1160 of 12497 results