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We narrowed to 6,962 results for: tet on

Showing: 1401 - 1450 of 6962 results
  1. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112668
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pLKO-Tet-puro-hRAF1-shRNA-1

    Plasmid
    #185371
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1
    Depositor
    Insert
    RAF1 (RAF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pLVX-TIGHT-CYCLINF-Tet-OFF

    Plasmid
    #32977
    Depositor
    Insert
    CYCLIN F (CCNF Human)
    Use
    Lentiviral
    Tags
    Flag and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    P-TIGHT
    Available since
    Feb. 13, 2012
    Availability
    Academic Institutions and Nonprofits only
  6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Insert
    dTET1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

    Plasmid
    #193348
    Purpose
    tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)
    Depositor
    Insert
    Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    TetO mini CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 1401 - 1450 of 6962 results