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pcDNA3-PM-TORCAR

Plasmid

#64930

Purpose

Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Publication

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

Lyn-TORCAR (EIF4EBP1 Human, Synthetic)

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

Plasmid

#44027

Depositor

Celeste Simon

Publication

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P405A, P530V, N851A

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMSCV-Flag-hKlf4

Plasmid

#20074

Depositor

Juan Belmonte

Publication

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hKlf4 (KLF4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pChREBP

Plasmid

#39235

Depositor

Isabelle Leclerc

Publication

da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

Insert

ChREBP (Mlxipl Mouse)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Publication

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Tags

Myc

Expression

Mammalian

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Publication

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
C-BLa 1.1

Plasmid

#88997

Purpose

BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

Depositor

Dieter Langosch

Publication

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

C-BLa 1.1 fusion protein

Tags

Flag

Expression

Bacterial

Promoter

araBAD

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1187 VSVGmut

Plasmid

#201913

Purpose

Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

VSV-G (K47Q, R354A)

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Publication

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-F-API5

Plasmid

#157662

Purpose

Expresses API5 in mammalian cell

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

FLAG tag

Expression

Mammalian

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pYES2-HTH

Plasmid

#86470

Purpose

Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter

Depositor

Akash Gunjan

Publication

Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

Type

Empty backbone

Tags

HIS10-TEV-HA

Expression

Yeast

Promoter

GAL1

Available since

Jan. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-hGSDMD

Plasmid

#111559

Purpose

Express human GSDMD in E.coli

Depositor

Hongbo Luo

Publication

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (GSDMD Human)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pGL4.23_Mmp11

Plasmid

#209520

Purpose

Expresses luciferase under control of Mmp11 promoter in transfected cells

Depositor

Jennifer Mitchell

Publication

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Mmp11 promoter (Mmp11 Mouse)

Use

Luciferase

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Human MAC-1 alpha

Plasmid

#8631

Depositor

Timothy Springer

Publication

Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

Insert

MAC-1 alpha (ITGAM Human)

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1/VChR1-EYFP

Plasmid

#20955

Purpose

Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

Depositor

Karl Deisseroth

Publication

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6 promoters

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJRH-1363 LV EF1a-CD19 IRES-EGFP

Plasmid

#201919

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD19 (CD19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Echovirus 11 (E11 3C) protease A isolate E11-PLA-NECK-23-E0-62-P1-FRA23

Plasmid

#228645

Purpose

Bacterial expression of the E11 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Echo virus 11 3C protease

Tags

His-SUMO

Expression

Bacterial

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMV-hAT-11_AMi

Plasmid

#217261

Purpose

Donor plasmid of hAT-11_AMi Transposon

Depositor

Yong E Zhang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of hAT-11_AMi transposon

Expression

Mammalian

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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