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MAPK11 gRNA (BRDN0001146070)

Plasmid

#77483

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK11

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK11 (MAPK11 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MK08_HUMAN_D0

Plasmid

#79695

Purpose

This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK08 (MAPK8 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK12 gRNA (BRDN0001146907)

Plasmid

#77485

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK12 gRNA (BRDN0001148543)

Plasmid

#77486

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK12 gRNA (BRDN0001147863)

Plasmid

#77487

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK12 (MAPK12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K3 gRNA (BRDN0001147239)

Plasmid

#76381

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K3 (MAP2K3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK10 gRNA (BRDN0001148192)

Plasmid

#75981

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK10 (MAPK10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K7 gRNA (BRDN0001149362)

Plasmid

#77820

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K7 gRNA (BRDN0001162319)

Plasmid

#77821

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K7 gRNA (BRDN0001149491)

Plasmid

#77822

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP2K7 gRNA (BRDN0001162371)

Plasmid

#77823

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSmEn2n4ERT(#234)

Plasmid

#184082

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2(n4)-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

Promoter

sCMV+SP6

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCSmEn2SRERT(#231)

Plasmid

#184080

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2(SR)-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

Promoter

sCMV+SP6

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22gCfpUbm5En2E4PCh(#251)

Plasmid

#184083

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm5Eng2bE4PCh(#284)

Plasmid

#184088

Purpose

cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22Ubm5Eng2aE4PCh(#283)

Plasmid

#184087

Purpose

cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2a-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSmEn25EERT(#232)

Plasmid

#184081

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2(5E)-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

sCMV+SP6

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK11 gRNA (BRDN0001147839)

Plasmid

#77484

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK11

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK11 (MAPK11 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DLG1 gRNA (BRDN0001148200)

Plasmid

#76617

Purpose

3rd generation lentiviral gRNA plasmid targeting human DLG1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DLG1 (DLG1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DLG1 gRNA (BRDN0001148018)

Plasmid

#76618

Purpose

3rd generation lentiviral gRNA plasmid targeting human DLG1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DLG1 (DLG1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge