We narrowed to 10,449 results for: T7

1. C413-E38: attB4-T7p>-attB1r

   Plasmid

   #162929

   Purpose

   Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   bacteriophage T7 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE-WT-CTGA-R13-AKSIRV

   Plasmid

   #63627

   Purpose

   CTGA-R13-AKSIRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   CTGA-R13-AKSIRV RNAP
   

   Use

   Tags

   n/a

   Expression

   Mutation

   V725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …

   Promoter

   T5/Lac

   Available since

   June 3, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pQE-WT-N4-R5-RNRV

   Plasmid

   #63630

   Purpose

   N4-R5-RNRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   N4-R5-RNRV RNAP
   

   Use

   Tags

   Expression

   Mutation

   L747I, N748D, L749C, M750V, F751I, Q754T, F755R, …

   Promoter

   T5/Lac

   Available since

   April 15, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pQE-WT-N4-R5-YRNRV

   Plasmid

   #63625

   Purpose

   N4-R5-YRNRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   N4-R5-YRNRV RNAP
   

   Use

   Tags

   Expression

   Mutation

   N671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …

   Promoter

   T5/Lac

   Available since

   June 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pQE-WT-CTGA-R13-AKSRV

   Plasmid

   #63622

   Purpose

   CTGA-R13-AKSRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   CTGA-R13-AKSRV RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

   Promoter

   T5/Lac

   Available since

   July 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTHSSd_44

   Plasmid

   #59966

   Purpose

   constitutive expression of T7 sigma fragment

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   T7 sigma fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Pcon sigma

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pARiBo4

   Plasmid

   #98893

   Purpose

   Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

   Depositor

   Pascale Legault

   Article

   Salvail-Lacoste et al RNA. 2013 Jul;19(7):1003-14. doi: 10.126

   Insert

   ARiBo4
   

   Use

   In vitro transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZB641_pT7_Lysin-KU1

   Plasmid

   #228511

   Purpose

   T7 RNAP inducible Lysin KU1 Expression

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   Lysin KU1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. C453-E26: attB4-T7p>-attB5

   Plasmid

   #162982

   Purpose

   Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   bacteriophage T7 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pN565

    Plasmid

    #49990

    Purpose

    expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    T7 RNAP*
    

    Use

    Synthetic Biology

    Tags

    umuD degradation tag

    Expression

    Bacterial

    Mutation

    R632S

    Promoter

    pTac-symmetric

    Available since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. 371-C

    Plasmid

    #97029

    Purpose

    His6-MBP-N10-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    His6 tag, MBP domain, N10 linker, and TEV cleavag…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-4F2-mCherry

    Plasmid

    #73421

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4F2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNSSCPG

    Plasmid

    #183187

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM6-3F2-mCherry

    Plasmid

    #73418

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3F2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-3A2-mCherry

    Plasmid

    #73425

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3A2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3A2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM6-5F5-mCherry

    Plasmid

    #73422

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 5F5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P5F5 (orthogonal T7-lac variant)

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM6-1E4-mCherry

    Plasmid

    #73426

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1E4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1E4 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-1B6-mCherry

    Plasmid

    #73420

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1B6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1B6 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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