We narrowed to 922 results for: cas12a

1. pMaCTag-20

   Plasmid

   #119999

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with 10xSuntag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   10xSuntag
   

   Use

   Pcr template

   Tags

   10xSuntag

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMaCTag-16

   Plasmid

   #119995

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with Dronpa3

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Dronpa3
   

   Use

   Pcr template

   Tags

   Dronpa3

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMaCTag-03

   Plasmid

   #119982

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with moxCerulean3

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   moxCerulean3
   

   Use

   Pcr template

   Tags

   moxCerulean3

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMaCTag-02

   Plasmid

   #119981

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with TagBFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   TagBFP
   

   Use

   Pcr template

   Tags

   TagBFP

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCH67 (crRNA expression vector)

   Plasmid

   #217337

   Purpose

   CROP-seq crRNA expression vector with GFP and puro marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRDA_887

   Plasmid

   #201164

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115211

   Purpose

   Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PsmCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCH49 (crRNA expression vector)

   Plasmid

   #217338

   Purpose

   CROP-seq crRNA expression vector with GFP and neo marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-neo
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMaCTag-P29

    Plasmid

    #120040

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115267

    Purpose

    Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    LbuCas13a
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115210

    Purpose

    Expresses AspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    AspCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AWP-029

    Plasmid

    #213966

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Synthetic, Segatella bryantii)
    

    Use

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDA_888

    Plasmid

    #201165

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-Z29

    Plasmid

    #120072

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AWP-031

    Plasmid

    #213967

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Synthetic, Segatella bryantii)
    

    Use

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ210

    Plasmid

    #109329

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-P26

    Plasmid

    #120037

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMaCTag-Z26

    Plasmid

    #120069

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMaCTag-P11

    Plasmid

    #120022

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet-i
    

    Use

    Pcr template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge