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We narrowed to 1,005 results for: control gRNA

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Showing: 561 - 580 of 1005 results

  1. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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  2. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

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  4. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106904-plasmid-map-sequence-id-206325

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  7. pJE53

    Plasmid
    #194153
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194153-plasmid-map-sequence-id-382435

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  9. pQdCas9.luxR(mut)-sggfp

    Plasmid
    #236185
    Purpose
    The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Promoter
    Quorum sensing promoter
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    236185-plasmid-map-sequence-id-471055

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  11. pYDE007

    Plasmid
    #194152
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194152-plasmid-map-sequence-id-382443

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  18. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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  19. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid
    #236039
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236039-plasmid-map-sequence-id-469911

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Showing: 561 - 580 of 1005 results