We narrowed to 15,732 results for: LEA

1. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

   Plasmid

   #102986

   Purpose

   AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   membrane-targeted SNAP protein after FLP-mediated recombination
   Nuclear-targeted eGFP after FLP-mediated recombination
   

   Use

   AAV; Adeno associated viral vector

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY005 (pcDNA3.1-hLb3Cpf1)

   Plasmid

   #69977

   Purpose

   Expresses humanized Lb3Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb3Cpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pRGEB-VirD2-Cas9

   Plasmid

   #139803

   Purpose

   Expressing VirD-Cas9 fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   VirD2-Cas9
   

   Tags

   VirD2-Cas9

   Expression

   Plant

   Promoter

   Ubiquitin

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. phFluc-WT

   Plasmid

   #177714

   Purpose

   Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

   Depositor

   Erik Böttger

   Article

   Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

   Insert

   hFluc WT
   

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXA4 (murine) HA-tag pET

   Plasmid

   #8489

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXA4 (Hoxa4 Mouse)
   

   Tags

   HA and T7

   Expression

   Bacterial

   Mutation

   Thr-2 changed to Ala-2 to form better ribosomal b…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pGEXNKI-his-GST-3C-LIC

   Plasmid

   #108709

   Purpose

   Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   GST-6xHis-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EYFP-NLS-actin-K68/284R

   Plasmid

   #60616

   Purpose

   Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

   Depositor

   Primal de Lanerolle

   Article

   Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

   Insert

   Beta-actin (ACTB Human)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   K68R, 284R

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLV-EF1a-Tmx4-V5

   Plasmid

   #120236

   Purpose

   Expresses V5-tagged Tmx4 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Tmx4 (Tmx4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. evoCas9

   Plasmid

   #138558

   Purpose

   Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   evoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF Doc2alpha WT GFP

    Plasmid

    #128819

    Purpose

    To visualize Doc2alpha WT in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK354

    Plasmid

    #129698

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120010799
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTU425

    Plasmid

    #178984

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT H585A C627A
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. tagSrc(WT)-mCer3 S208F/V224L

    Plasmid

    #179138

    Purpose

    mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA
    

    Tags

    mCerulean3 (S208F/V224L)

    Expression

    Mammalian

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV V5-Vkorc1l1

    Plasmid

    #175148

    Purpose

    Lentiviral expression of V5-tagged mouse Vkorc1l1

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Vkorc1l1 (Vkorc1l1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-miniSOG-VAMP2-citrine

    Plasmid

    #50969

    Purpose

    AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    miniSOG-VAMP2-citrine (Vamp2 Mouse, Arabdopsis thaliana)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_EE-nCas9

    Plasmid

    #163541

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-STChRger2-TS-EYFP

    Plasmid

    #129395

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PD-L1-miSFIT-1x

    Plasmid

    #124685

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NG0750 pCI-TPI-PTC48-4H

    Plasmid

    #65803

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    premature stop codon at 48

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. JPF0419d

    Plasmid

    #124020

    Purpose

    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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