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pEY81

Plasmid

#191076

Purpose

flp-32 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-32 (flp-32 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY75

Plasmid

#191071

Purpose

cng-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cng-3 (cng-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY67

Plasmid

#191064

Purpose

ceh-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-1 (ceh-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY69

Plasmid

#191066

Purpose

ceh-24 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-24 (ceh-24 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186708

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Promoter

PpsbA2*

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186710

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Promoter

Ptrc.x.tetO2

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA

Plasmid

#186411

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA

Plasmid

#186408

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP

Plasmid

#186412

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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PDEST-pSP172BSSPE-pFOGc::RfA-ECFP

Plasmid

#186403

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA-ECFP

Plasmid

#186402

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::RfA-venus

Plasmid

#186401

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::venus-RfA

Plasmid

#186400

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::venus-RfA

Plasmid

#186398

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-NLSlacZ-stop-L2

Plasmid

#186358

Purpose

Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of nuclear lacz reporter

Tags

Nuclear localization signal

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ZmIAA29 (yeast codon optimized)

Plasmid

#188441

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA29 (yeast codon optimized)

Use

Cloning

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA-HA

Plasmid

#186405

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus

Plasmid

#186409

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA

Plasmid

#186410

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Cherry-Plekhh1 5 silent mutations

Plasmid

#187373

Purpose

Expresses human Plekhh1 with 5 silent mutations labelled with Cherry

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1 with 5 silent mutations

Tags

mCherry

Expression

Mammalian

Mutation

Five silent mutations at the Plekhh1 siRNA site (…

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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