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pPLtetO-FtsA

Plasmid

#107558

Purpose

Plasmid for titrating synthesis of FtsA with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

ftsA

Use

Synthetic Biology

Promoter

pLtetO

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 0.6kb promoter

Plasmid

#20126

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 MDM2 S166D

Plasmid

#16234

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser166 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pMRXIP Lamp1-Venus

Plasmid

#89937

Purpose

Expresses Lamp1 tagged with Venus in mammalian cells

Depositor

Noboru Mizushima

Article

Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

Insert

rat Lamp1 (Lamp1 Rat)

Use

Retroviral

Tags

Venus

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-mTSP33

Plasmid

#236541

Purpose

For expression of Tspan33 in mammalian cells

Depositor

Sandra Citi

Article

Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

Insert

TSPAN33 (Tspan33 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-memRFP-3xPax7gRNA

Plasmid

#224569

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

CRISPR

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsRRP12_K

Plasmid

#146766

Purpose

Mammalian Expression of HsRRP12

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12 (RRP12 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-30-RexYFP

Plasmid

#141070

Purpose

Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

RexYFP

Tags

6xHis

Expression

Bacterial

Mutation

changed Leucine 169 to Proline, changed Tyrosine …

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Expression

Insect

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAT9650-BEAR-GFP-preedited

Plasmid

#162992

Purpose

BEAR control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsZ

Plasmid

#89656

Purpose

Plasmid for titrating synthesis of FtsZ with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

GTP-binding tubulin-like cell division protein

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDONOR-tagBFP-PSM-EGFP

Plasmid

#100603

Purpose

Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Type

Empty backbone

Use

Synthetic Biology

Tags

EGFP-T2A-Puro and tagBFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Consensus

Plasmid

#176678

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIB_P2A_Hygro_Barcode

Plasmid

#120483

Purpose

Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIB (SPIB Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLN382 (FuGW-S(HIF1A)p-mKate2 )

Plasmid

#105180

Purpose

synthetic promoter HIF1A drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(HIF1A)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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