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pDONR223_NRAS_WT

Plasmid

#81901

Purpose

Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PEX3_WT

Plasmid

#81779

Purpose

Gateway Donor vector containing PEX3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PEX3 (PEX3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_WT

Plasmid

#81755

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_WT

Plasmid

#81737

Purpose

Gateway Donor vector containing PTEN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CB_WT

Plasmid

#82221

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRS1_WT

Plasmid

#82214

Purpose

Gateway Donor vector containing IRS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRS1 (IRS1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225239

Purpose

Mammalian expression of iPASnFR-H in mitochondria

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pACS131

Plasmid

#204596

Purpose

Histone H3.1K27M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 K27M (H3C1 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 K222R

Plasmid

#80887

Purpose

mammalian expression of ALK7 K222R

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Tags

HA

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-Casp2

Plasmid

#50023

Purpose

pENTR vector for mouse Caspase-2

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Casp2 (Casp2 Mouse)

Use

Gateway Cloning

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
pKD001 pME minprom_EGFP

Plasmid

#195955

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann

Article

Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

Insert

mouse beta-globin minimal promoter EGFP

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF15_P2A_Hygro_Barcode

Plasmid

#120501

Purpose

Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF15 (KLF15 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF5_P2A_Hygro_Barcode

Plasmid

#120491

Purpose

Barcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF5 (KLF5 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA3_P2A_Hygro_Barcode

Plasmid

#120440

Purpose

Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA3 (FOXA3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

Plasmid

#111744

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q30 (polyQ repeat)

Promoter

CMV and P10

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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ACO2_pLX307

Plasmid

#98312

Purpose

Lentiviral expression of ACO2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ACO2 (ACO2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4254

Plasmid

#89050

Purpose

Expresses St chimeric gRNA and human codon-optimized StCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

St chimeric gRNA
hStCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

Plasmid

#83491

Purpose

cre-dependent DN-AMPK under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

DN-AMPK-2A-mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

K45R

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-ABCB10-ChR2-eYFP

Plasmid

#206037

Purpose

Express ChR2 in mitochondria

Depositor

Lufang Zhou

Article

Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

Insert

ChR2

Tags

ABCB10 mitochondrial leading sequence and EYFP

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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