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GB4829

Plasmid

#215275

Purpose

TU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:NPGA:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-FRB

Plasmid

#194455

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with FRB fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FRB (MTOR Human)

Tags

GAG

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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GB4832

Plasmid

#215276

Purpose

Module for the constitutive expression of HISPS, H3H, EGFP and p23.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

HISPS-H3H-EGFP-p23

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-APP-CTF

Plasmid

#181732

Purpose

Galactose inducible expression of APP-CTF

Depositor

Alejandro Chavez

Insert

APP-CTF (APP Human)

Expression

Yeast

Mutation

C-terminal fragment (aa 671-770)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(R150A)-3xFLAG-pCDNA

Plasmid

#201966

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R150A

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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SGTA-2Strep

Plasmid

#210353

Purpose

Expresses SGTA fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SGTA (SGTA Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FB395

Plasmid

#203631

Purpose

TU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1aG2b.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB396

Plasmid

#203632

Purpose

TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1ab.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB397

Plasmid

#203633

Purpose

TU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1abc.3:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1C1

Plasmid

#201730

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1C1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1C1

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1A2

Plasmid

#201729

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A2 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A2

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1A1

Plasmid

#201728

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A1

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-eDHFR

Plasmid

#194635

Purpose

Mammalian expression of the eDHFR control bait in Virotrap

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

ecDHFR

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 1

Plasmid

#154253

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 1 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 2

Plasmid

#154254

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 2 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 3

Plasmid

#154255

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 3 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E21: attB4-FerHp>-attB5

Plasmid

#162978

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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