164,802 results

1. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

   Plasmid

   #172627

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgStk11#2/Cre

   Plasmid

   #173653

   Purpose

   Expresses a Stk11-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Stk11 (Stk11 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_256

   Plasmid

   #158581

   Purpose

   Lentiviral expression of BE3.9max and customizable gRNA

   Depositor

   David Root, John Doench

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRE-Tn5-157

   Plasmid

   #118539

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mClover3
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PKCalpha.FLAG

   Plasmid

   #10805

   Depositor

   Alex Toker

   Article

   Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

   Insert

   PKC alpha (PRKCA Bovine)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D332G-please see comments below

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. FKBP1B

   Plasmid

   #73241

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FKBP1B (FKBP1B Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Wnt7A

   Plasmid

   #35914

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt7A (WNT7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV4 p50

   Plasmid

   #21965

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NF-kB p50 subunit (NFKB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2/LTR7-GFP

    Plasmid

    #62541

    Purpose

    LTR7-driving reporter

    Depositor

    Zsuzsanna Izsvak

    Article

    Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

    Insert

    LTR7-GFP
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR7

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Brm WT-Flag pShuttle

    Plasmid

    #122105

    Purpose

    Expression of full length Brm in mammalian cells

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    BRM (SMARCA2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LD-RA

    Plasmid

    #209863

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Use

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0861

    Plasmid

    #141740

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1H4 (NR1H4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SIRT3L-12

    Plasmid

    #13736

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT3 (SIRT3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human Myc-XIAP

    Plasmid

    #217627

    Purpose

    Mammalian expression of Myc tagged XIAP

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.DEST.YFP

    Plasmid

    #32683

    Purpose

    Lentiviral cDNA/shRNA gateway destination vectors

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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