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1478 pcDNA3 flag HA 14-3-3 beta

Plasmid

#8999

Depositor

William Sellers

Insert

14-3-3 beta (YWHAB Human)

Tags

HA and flag

Expression

Mammalian

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Publication

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171379

Purpose

Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Publication

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized Cas13X.1

Expression

Mammalian

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Publication

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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VRQR-ABEmax

Plasmid

#119811

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6 promoters

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Publication

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM444

Plasmid

#108319

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGL3 2 kb prom. CD274

Plasmid

#107003

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Publication

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Publication

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAW63.YY1.FKBP.knock-in.BFP

Plasmid

#104371

Purpose

For knocking in FKBP F36V P2A BFP

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-BFP

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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-5.5kb-UCP1-GFP

Plasmid

#104585

Purpose

Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

Depositor

Irfan Lodhi

Publication

Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

Insert

UCP1 promoter (Ucp1 Mouse)

Tags

TurboGFP

Expression

Mammalian

Promoter

UCP1

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLJC5-LAMP1-RFP-2xFlag

Plasmid

#102931

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

Depositor

David Sabatini

Publication

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

mRFP-2xFlag

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSNAP-tag (T7) Vector

Plasmid

#101137

Purpose

Expression of protein of interest with SNAP-Tag in bacterial cells

Depositor

New England Biolabs, Ana Egana

Type

Empty backbone

Tags

SNAP-tag (SNAP26b)

Expression

Bacterial

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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B52 (empty plasmid backbone to express 2 sgRNAs)

Plasmid

#100708

Purpose

Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

Depositor

Alberto Ciccia

Publication

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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DNA-PKcs T3950D

Plasmid

#83320

Purpose

DNA-PKcs T3950A

Depositor

Katheryn Meek

Publication

Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

Activation loop phosphorylation site 3950 substit…

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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