We narrowed to 12,203 results for: 112

1. pDNR-mCherry-T2A-3xPuroR-Frame 0

   Plasmid

   #206991

   Purpose

   Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-U6insert-EF1puro

   Plasmid

   #104537

   Purpose

   A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

   Depositor

   George Church

   Article

   Kalhor et al Science. 2018 Aug 9. pii: science.aat980

   Type

   Empty backbone

   Use

   CRISPR, Mouse Targeting, and Synthetic Biology ; …

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS159

   Plasmid

   #122378

   Purpose

   CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCap-SG5-Hgy

   Plasmid

   #227580

   Purpose

   Capture plasmid with hygromycin resistance and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF525-EF1a-Hygro-P2A-mCherry-lenti

   Plasmid

   #115796

   Purpose

   Expresses mCherry in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   EF-1a promoter

   Available Since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LM670

   Plasmid

   #191552

   Purpose

   LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

   Depositor

   David Baker

   Article

   Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

   Insert

   ccdb
   

   Tags

   HIS-tag

   Expression

   Bacterial

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNR291

   Plasmid

   #79555

   Purpose

   Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   GFPmut3b
   mTagBFP
   mRFP
   

   Expression

   Bacterial

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM446 (Flag-DAS+4))

   Plasmid

   #108321

   Purpose

   Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Flag-DAS+4
   

   Use

   Orbit integrating plasmid

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. HLA_A2402

   Plasmid

   #165010

   Purpose

   Expression of HLA-A*24:02 cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-HA-UBA6

    Plasmid

    #136995

    Purpose

    Expresses human UBA6 in mammalian cells

    Depositor

    Marcus Groettrup

    Article

    Pelzer et al J Biol Chem. 2007 Aug 10;282(32):23010-4

    Insert

    UBA6 (UBA6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPP441

    Plasmid

    #158801

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Tags

    2xSV40NLS-2xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR2b-mTurquoise2

    Plasmid

    #191014

    Purpose

    Gateway entry vector with the mTurquoise2 fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

    Insert

    mTurquoise2
    

    Use

    Gateway entry vector

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-RFP-shCntrl

    Plasmid

    #69040

    Purpose

    Lentiviral expression of control shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    control shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDNR-mCherry-T2A-3xPuroR-Frame 1

    Plasmid

    #206992

    Purpose

    Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDNR-mCherry-T2A-3xPuroR-Frame 2

    Plasmid

    #206993

    Purpose

    Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSJ95

    Plasmid

    #104343

    Purpose

    Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Chen et al Science. 2017 Jan 27;355(6323). pii: 355

    Insert

    FBP1
    

    Tags

    3xFLAG

    Expression

    Yeast

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPP085

    Plasmid

    #158795

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mpeg:lamp1b-mCherry-stop; cmlc2:GFP

    Plasmid

    #213739

    Purpose

    Expresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

    Depositor

    William Talbot

    Article

    Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

    Insert

    lamp1b-mCherry
    

    Use

    Zebrafish

    Promoter

    mpeg1.1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCBI-CRY2PHR-VPR

    Plasmid

    #192606

    Purpose

    Plasmid for constitutive expression of CRY2PHR-VPR.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    CRY2PHR-VPR
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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